EPF-Lausanne/26 August 2009

Wet Lab

Culture

The following bacteria have been cultivated and analyzed by the fluorescent microscope in Sebastian's lab : 3x RO2 (#4,5,10), 4x RO2-BB(#4-1/#5-1/#8-5/#10-6), 3x RO2 (#4,5,10).

Colony PCR

The result of the double transformation of yesterday gave the following number of clones :

RO2#10 - BB6 : ~ 30 RO2#8 - BB5 : ~ 30 RO2#5 - BB3 : a lot RO2#4 - BB1 : a lot

There has been a double antibiotic resistance so there might be a good chance these bacteria have included both plasmids. To be sure of it, we did a colony PCR.

Cloning

PCR

Digestion

Purification

Ligation

Transformation

Migration

People in the lab

Basile, Rafael, Nicolas