From 2009.igem.org
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December 2008
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March 2009
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April 2009
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May 2009
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November 2009
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Week from May 25th, to May 31st, 2009
May, 25th
- We planned to dedicate the first two weeks to prepare a glycerol stock for every BioBrick of interest for our project. The following weeks will be dedicated to the BioBrick assembly.
- DNA resuspension from iGEM 2009 plates. We resuspended all the high quality constitutive promoter family members from UCBerkeley collection and GFP protein generator:
J23100
| J23101
| J23102
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J23103
| J23104
| J23105
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J23106
| J23110
| J23112
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J23113
| J23114
| J23116
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J23117
| J23118
| E0240
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- Transformation of resuspended DNA (2 ul) in TOP10 E. coli, plated transformed bacteria and incubated the plates overnight at 37°C.
May, 26th
- All the 15 incubated plates showed colonies!
Moreover, many UCBerkeley promoter family members showed red fluorescent colonies, according to the ranking of promoters' estimated strength (see http://partsregistry.org/Promoters/Catalog/Anderson).
High quality Berkeley promoters and E0240 plates: high yield for all of them!
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J23102 plate: the promoter is very strong and RFP expression could be seen in the red-coloured colonies. The same result was seen in the other strong Berkeley promoters
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- We picked one colony from each plate and infected 1 ml of LB + Amp. We incubated the inocula at 37°C, 220 rpm for 5 and 1/2 hours.
- After 5 hours and 1/2 all the cultures were turbid and bacteria were in their exponential growth phase.
- Glycerol stocks for the grown cultures.
May, 27th
Experiment with Tecan F200
- Description
- Purpose:
- Materials & Methods
- Protocol
- Results
May, 28th
May, 29th
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