Team:EPF-Lausanne/Results/Fusion
From 2009.igem.org
DNA-binding domain
The first step in our computational study of the LOV domain was to fuse the 2 domains of interest in VMD. We were then able to visualize the different proteins tried by Sosnick. The working protein, that we call LovTAP is the result of the fusion at PHE22 of trpR and can be seen on the next video. The general LOV domain is in yellow. Please note the chromophore called Flavin (FMN) in red in the center of LOV2. The trpR dna binding domain is in orange and DNA in gray.
Click here to see an example of code used in VMD for the fusion
We also modelized the other fusion tried by Sosnick.
- @MET11
- @ALA12
- @GLU13
- ...
- @PHE22
- ...
- @LEU25