Team:Newcastle/Meetings/16 March 2009

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Contents

16/03/2009 - iGEM Meeting

Agenda of the Meeting (16/03/09)

  • Come up with the project title
  • Create the frontpage of the wiki
  • Discuss our project title (combination of the 1st and 3rd idea)
  • Come up with the paragraph summarising our project idea to be put up on the external wiki


Agenda of the Meeting (18/03/09)

  • Introduce ourselves to Nigel Robinson
  • Review last meetings action points
  • Present our ideas in the form of a flowchart/movie clip
  • Feedback and discussion
  • Questions
  • Show paragraph summarising our idea
  • Review additional things


NEW Action Points From Last Meeting

  • Review the questions and challenges put forward for each idea
  • Do additional reading/research into ideas but more of an emphasis on the first and third ideas
  • Finalise an idea and write a paragraph on the idea to be posted in the main wiki
  • Reschedule wiki tutorial with Morgan.


Minutes of the Meeting (16/03/09)

  • Start: 5p.m at Claremont Tower Room 601, End: 7p.m
  • Reviewed previous actions points
    • Funding - Done (Jessica)
  • Briefly discussed about BioBricks
  • Discussed about the awarding of the medals
    • Our project would probably be based on the environmental side
    • Some of the "easier" awards which can be more "easily" obtained are: Best Wiki, Best Poster, Best Environmental Project, Best Presentations, etc.
    • One of the criteria for the medal is to help a team, helping a new team would probably be easier.
  • To make our wiki more attractive, a banner using flash, javascript would be desired, referred to iGEM2008 Heidelberg's wiki as a good reference of a nice wiki.
  • Brainstormed on the paragraph on our idea to be put on the external wiki.
  • Brainstormed for our title, coming up with keywords such as
    • Heavy metals
    • Stochastic switch
    • Soil/mud
    • Tunable
    • Sporulation
    • Sequester
    • Spores
    • Thousand years memory
    • Scavenge
    • Hunter
    • Seeker
    • Basillus Subtillis
    • Kamikaze/Suicde
    • The Quest to Sequester
  • Discussed that, perhaps, with the combination of the two ideas, could just use UV light to shine to "test" the sample, instead of having to take a sample and bringing it back to the lab for testing.
  • Discussed that antibodies could be used in identifying the spores.
  • Questions
    • How to extract the metals from the cell?
    • How can we get metalloproteins into the cells?
    • Sporulation is induced due to stress, how much metal can we get into the cell?


Action Points of the Meeting (16/03/09)

  • Familiarise self with BioBricks, website to go: www.partsregistry.org - ALL
  • Craig, Matthew, Arun to come up with the presentation slides, with the addition of pictures, flow diagrams, instead of just words to present the idea.
  • Read Nigel Robinson's paper (11 pages) in preparation for Wednesday's (18/03/09) meeting - ALL
  • Think of questions to ask regarding Nigel Robinson's paper - ALL


Minutes of the Meeting (18/03/09)

  • Craig, Matthew and Arun presented our project idea to the team and guest advisor Nigel Robinson
  • Nigel Robinson posted some questions
    • What metals are we interested in?
      • Cadmium, Mercury, Arsenic >> Red List metals
      • Will be difficult to import the red list metals, normally imports Mg, Zn, etc instead and not the red list metals >> Switch the exporter switch for Cd efflux
      • E.Coli has a mercury importer on ...
      • Silver will go in through copper??
      • Importer for Zn in Bacillus, express a protein ...
      • Put Gsh A, Gsh B into ... (from E.Coli)
      • Knock out Cad A, then find the maximum Cad A that survived.


Action Points of the Meeting (16/03/09)

  • List all the BioBricks needed
  • Develop software systems which stimulates the system
  • X-Mind (?)



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