Uppsala-Sweden/10 October 2009
From 2009.igem.org
Plasmid prep
A plasmid prep was performed for PRPRYT 3 & 4 on pSB1AK3.
Triparental mating
BG-11 plates were made without antibiotics and without CuSO4 to have the pPetE promoter as silent as possible during triparental mating.