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• Metal Intake/Efflux
• Metal Sensing
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Metal Sensing

Introduction

If our project is to process cadmium and not other metals, we need to genetically engineer Bacillus subtilis to carry out a set of cellular processes based on the action of metal sensors. These metal sensors will detect cadmium through a system known as AND Gating.

There are two metal sensing repressors, which are known to respond to cadmium: arsR and czrA. By placing binding sites to these two metal sensing repressors next to each other in a promoter region, the gene regulated by that promoter will be synthesized only when a combination of metals that bind to both sensors are present; this is a combinatorial approach for gene expression regulation.

Modelling

• Metal Intake/Efflux Model

BioBrick constructs

Lab Work Strategies

• Metal Sensor: czrA+arsR - plan A

• Metal Sensor: czrA+arsR - plan B

Other Presentations and Diagrams

Lab Work done

Summary of Lab Sessions for Cadmium Sensing
Date	Description
18th August 2009	Transformed DH5-alpha E. coli cells with BBa_J33206 from the Spring Distribution
19th August 2009	Inoculated 3 tubes of LB with 3 colonies of potential transformant E.coli cells