Team:Newcastle/Project/Labwork/OurProtocols/PCR Products

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Meetings Calendar

Use PCR Clean-up kit to clear the PCR product

This should result in 50ul of final product

Measure the concentration of the product

This step will will be useful to determine how much of the product to use for the ligation

Digest the PCR product

Although the total will be 50ul, some of it will be consumed for the gel and to measure the concentration. Make a total solution of 70ul using the followings. (Check the restriction sites and replace EcoRI and SpeI as you need)

- 50ul of the PCR product
- 3ul of EcoRI
- 3ul of SpeI
- 7ul of 10X buffer
- Use water to make the final volume 70ul

Mix everything well and incubate at 37C for an hour

20 – 21 June 2009 - Europe workshop (London)
23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
23 October Practice Presentation (Newcastle)
23 October T-shirts are ready
27 October Practice Presentation (Sunderland)
27 October Poster is ready
30 October – 2 November 2009 - Jamboree (Boston)

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