Team:Newcastle/Labwork/28 July 2009

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Revision as of 22:58, 28 September 2009 by MJR09 (Talk | contribs)

Lab Work - 28/07/09

Introduction

In the past few lab sessions the team has transformed JM109 E. coli cells with five BioBricks from the Spring Distribution and attempted to grow the transformant cells on LB + amp plates. The five BioBricks include BBa_C0056 (cI in the lambda phage), BBa_B1002 (terminator sequence), BBa_C0077 (CinR activator), BBa_C0076 (autoinducer synthetase) and BBa_R0077 (promoter which is CinR and HSL regulated - RBS+).

It was found that when the five sets of transformant E. coli cells were plated out, the cells possessing BBa_C0056, BBa_B1002, BBa_C0076 and BBa_R0077 seemed to produce colonies (although it must be noted that there was a little uncertainty about whether the E. coli transformed with BBa_C0076 did actually produce colonies). Three colonies from each of the four transformant plates were taken and each used to inoculate tubes containing 5ml LB solution (making a total of 12 tubes). These tubes were then placed in the orbital incubator overnight for mini-preps.

Today's plan is to conduct mini-preps on the four sets of E. coli transformant cells

Practical Outline

Here's the objectives for today's lab session:

  1. Carry out mini-preps of the E. coli cells which have taken up the BioBricks BBa_C0056, BBa_B1002, BBa_C0076 and BBa_R0077



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