EPF-Lausanne/3 September 2009

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==Wet Lab==
==Wet Lab==
 +
* '''Results of the RO1:'''
 +
There is clones on every plate!
 +
: → colony PCR on all clones with the Taq Platinium protocol
 +
<br>
 +
* '''Results from the media try:'''
 +
All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture.
 +
{| class="wikitable" style="text-align:center; width:80%;"
 +
|-
 +
! scope=col |
 +
! scope=col | RO2 #6
 +
! scope=col | RO2 #7
 +
! scope=col | RO2 #8
 +
|-
 +
! scope=row | M9/min + AA + glycerol
 +
|white
 +
|white
 +
|white
 +
|-
 +
! scope=row | LB
 +
|LB + slight red
 +
|LB + red
 +
|LB
 +
|-
 +
! scope=row | M9/min + AA + thiamine
 +
|rose
 +
|white
 +
|white
 +
|-
 +
! scope=row | M9/min + thiamine
 +
|rose
 +
|rose
 +
|white
 +
|-
 +
! scope=row | SC
 +
|NA
 +
|NA
 +
|NA
 +
|}
 +
 +
* '''Culture for characterization'''
 +
We tried the following media, with and without Trp :
 +
: LB
 +
: M9/min + thiamine
 +
: M9/min + AA + glycerol
 +
: M9/min + thiamine
 +
 +
The gel showed :
 +
: '''RO1''': it works fine, all our constructs have the TrpO
 +
: '''RO2''': #6 and #7 do not have the construct (we took the wrong clones), while #8 seems to have the construct!
==People in the lab==
==People in the lab==

Latest revision as of 14:32, 8 September 2009



Contents

3 September 2009




Wet Lab

  • Results of the RO1:

There is clones on every plate!

→ colony PCR on all clones with the Taq Platinium protocol


  • Results from the media try:

All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture.

RO2 #6 RO2 #7 RO2 #8
M9/min + AA + glycerol white white white
LB LB + slight red LB + red LB
M9/min + AA + thiamine rose white white
M9/min + thiamine rose rose white
SC NA NA NA


  • Culture for characterization

We tried the following media, with and without Trp :

LB
M9/min + thiamine
M9/min + AA + glycerol
M9/min + thiamine

The gel showed :

RO1: it works fine, all our constructs have the TrpO
RO2: #6 and #7 do not have the construct (we took the wrong clones), while #8 seems to have the construct!

People in the lab

Basile, Mélanie, Caroline



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