Team:Aberdeen Scotland/Ourparts

BBa_K182001 - Intermediate Part consisting of LacI ( RBS+, Term +) and a Tet operator.
This is an intermediate part using the biobrick BBa_I732820 (RBS, LacI, Term) upstream and the Biobrick BBa_R0040 (TetR repressible promoter) downstream.
It comes with a ribosome binding site upstream of LacI and a Terminator downstream (BBa_B0015) There is no Promoter upstream of LacI, meaning that LacI will not be expressed in this piece.
The TetR repressible promoter BBa_R0040 is downstream of the Terminator.

BBa_K182005
This is a part using the biobrick BBa_K182004 as the upstream part and BBa_S03518 as the downstream part.
BBa_K182004 is a cI regulated promoter. The promoter has two binding sites for the cI repressor and is repressed when it binds.It is identical to BBa_R0051 apart from a point mutation in the -35 promoter binding region(15th base is C instead of T).
BBa_S03518 is a TetR protein with a Ribosome Binding site upstream and an LVA tag attached to its end. TetR binds to TetR regulated promoters such as BBa_R0040. ATC (anhydrotetracycline) binds to TetR causing it to be released from the promoter and reintroducing transcription.
This part comes without a terminator.

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