Team:EPF-Lausanne

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{|
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{{EPF-Lausanne09}}
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<div CLASS="epfl09front">
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[[Image:Leman-dents-du-midi2.jpg]]
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[[Image:Mainpage.jpg|center]]
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<div CLASS="epfl09bis">
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<br><br>
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==Concept==
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<br>
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<div style="text-align:justify;">
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[[Image:LovTAP_dimer.png|right|300px|thumb|LovTAP dimer bound to DNA]]
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Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.
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For this purpose, we used a light-sensitive DNA binding protein "LovTAP" (for Light, Oxygen, Voltage Tryptophan-Activated Protein) to convert a light input into a chosen output, here fluorescence generated by the RFP reporter gene.
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| align="center" textcolor="white" |[[Team EPF-Lausanne]]
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<!--- The Mission, Experiments --->
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The results clearly show that this light-induced gene switch tool works ''in vivo'', demonstrating the feasibility of implementing such powerful technology for a diverse range of bio(techno)logical applications.
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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</div>
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!align="center"|[[Team:EPF-Lausanne|Home]]
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!align="center"|[[Team:EPF-Lausanne/Team|The Team]]
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<br><br><br><br>
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!align="center"|[[Team:EPF-Lausanne/Project|The Project]]
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{| class="wikitable" style="text-align:center; width:100%;"
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!align="center"|[[Team:EPF-Lausanne/Parts|Parts Submitted to the Registry]]
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|-
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!align="center"|[[Team:EPF-Lausanne/Modeling|Modeling]]
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! scope=col | [[Image:Logo_MerckSerono.png|80 px]]
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!align="center"|[[Team:EPF-Lausanne/Notebook|Notebook]]
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! scope=col | [[Image:Logo_Novartis.png|100 px]]
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!align="center"|[[Team:EPF-Lausanne/Lectures|Lectures]]
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! scope=col | [[Image:Logo_Syngenta.png|80 px]]
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!align="center"|[[Team:EPF-Lausanne/Team Management|Team Management]]
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! scope=col | [[Image:Logo_UBS.jpg|200 px]]
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! scope=col | [[Image:Logo_ciba.jpg|80 px]]
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! scope=col | [[Image:Logo_nikon.jpg|60 px]]
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! scope=col | [[Image:Logo_tecan.gif|80 px]]
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|}
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(''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')
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----
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----
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</div><div CLASS="epfl09bouchon"></div>

Latest revision as of 22:45, 21 October 2009

Mainpage.jpg



Concept


LovTAP dimer bound to DNA

Recent discoveries of photoreceptors in many organisms got us excited about the possibility of using light-responsive genetic tools in synthetic biology. Indeed, such tools could in principle induce phenotypic changes in a more localized, preciser and faster fashion than currently available chemical-based methods. To evaluate the biotechnological potential of such tools, we specifically aimed to induce a change in gene expression, more specifically to directly turn a gene on or off, in a living organism, in response to a light stimulus.

For this purpose, we used a light-sensitive DNA binding protein "LovTAP" (for Light, Oxygen, Voltage Tryptophan-Activated Protein) to convert a light input into a chosen output, here fluorescence generated by the RFP reporter gene.

The results clearly show that this light-induced gene switch tool works in vivo, demonstrating the feasibility of implementing such powerful technology for a diverse range of bio(techno)logical applications.





Logo MerckSerono.png Logo Novartis.png Logo Syngenta.png Logo UBS.jpg Logo ciba.jpg Logo nikon.jpg Logo tecan.gif

Locations of visitors to this page