Team:EPF-Lausanne

From 2009.igem.org

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(Last News)
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'''Keep track with what we did so far'''
'''Keep track with what we did so far'''
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 +
(12.07.09)
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:This first week of wetlab we have done the following things
 +
*Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
 +
*Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an incducible promoter (LacI) (+RBS +term.)
 +
*Orderd and received the primers needed for the PCR of LovTAP
 +
*Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
 +
*Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about this parts) into competent E.Coli
 +
*Fused the two BioBricks LacI and term
 +
*Digested the LovTAP PCR product and RBS part

Revision as of 15:43, 12 July 2009

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Last News

Keep track with what we did so far

(12.07.09)

This first week of wetlab we have done the following things
  • Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
  • Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an incducible promoter (LacI) (+RBS +term.)
  • Orderd and received the primers needed for the PCR of LovTAP
  • Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
  • Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about this parts) into competent E.Coli
  • Fused the two BioBricks LacI and term
  • Digested the LovTAP PCR product and RBS part




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