Team:EPF-Lausanne/Last News

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Revision as of 13:15, 29 July 2009



Contents

<!- lov lov2 --->




Last News



Keep track with what we did so far

(12.07.09)

This first week of wetlab we have done the following things
  • Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
  • Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
  • Ordered and received the primers needed for the PCR of LovTAP
  • Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
  • Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
  • Fused the two BioBricks "LacI" and "RBS"
  • Digested the LovTAP PCR products and RBS part


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