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Team:EPF-Lausanne/Last News
From 2009.igem.org
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Revision as of 13:16, 29 July 2009
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Keep track with what we did so far
(12.07.09)
- This first week of wetlab we have done the following things
- Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
- Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
- Ordered and received the primers needed for the PCR of LovTAP
- Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
- Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
- Fused the two BioBricks "LacI" and "RBS"
- Digested the LovTAP PCR products and RBS part
