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Team:Imperial College London/Temporal Control
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*[http://en.wikipedia.org/wiki/Green_fluorescent_protein GFP] is a green coloured flourescent protein that is also commonly used as a reporter. The coding gene is under control of the same promoter as the genes for Module 2. This means that GFP expression is tied into the module, and we can see the rise in GFP levels that correlate with the switch to the secondary carbon source and therefore the start of Module 2. The GFP again must be normalised against optical density (shown above) to account for the increase in cell density.--> | *[http://en.wikipedia.org/wiki/Green_fluorescent_protein GFP] is a green coloured flourescent protein that is also commonly used as a reporter. The coding gene is under control of the same promoter as the genes for Module 2. This means that GFP expression is tied into the module, and we can see the rise in GFP levels that correlate with the switch to the secondary carbon source and therefore the start of Module 2. The GFP again must be normalised against optical density (shown above) to account for the increase in cell density.--> | ||
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| - | + | and [[Team:Imperial_College_London/Temporal_Control/Graph#Testing_Construct | click here]] to see our testing construct. | |
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| - | [[Team:Imperial_College_London/Temporal_Control/Graph#Testing_Construct | | + | |
===Results=== | ===Results=== | ||
Revision as of 11:22, 17 October 2009
Contents |
Temporal Control
Engineering Approach to Temporal Control
This temporal control platform showcases our engineering approach in the E.ncapsulator project. We’ve made our entire system modular. Each module can essentially be pictured as a blackbox with one temporal control input and one output. Therefore, each module is linked to the next module by temporal control. Temporal control has allowed us to create a system that can be reused in other projects in synthetic biology. It is in fact a novel engineering approach that is both reusable and elegant.
In our temporal control system, we have employed 3 kinds of temporal control:
- Chemoinduction:
Production of the protein of interest is triggered by the addition of a chemical.
In our case, we have chosen IPTG. IPTG will be added when the cell density is deemed sufficient for protein production (Module 1) to begin.
- Autoinduction:
When glucose levels have fallen to nearly 0, encapsulation (Module 2) will begin automatically in response.
In our case, this will allow a sufficient amount of protein production to have taken place, before the cell focuses its resources on encapsulation.
- Thermoinduction:
Genome deletion is triggered by the increase of temperature. This is the last step of the temporal control system.
In our case, thermoinduction was necessary, as chemical induction may be blocked by the presence of the capsule (that inhibits diffusion).
Timeline of Temporal Control
This timeline shows the sequence of occurrence of these events:
about the timeline and its explainations!
and click here to see our testing construct.
Results
Project Tour
For more details of the temporal control of the system, see the tabs below.
Temporal Control Contents
