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Revision as of 23:46, 18 August 2009

Meetings Calendar

Lab 18/08/09

Sporulation Tuning/ Chassis Team

Summary

Today, the moment of truth, the recovery of spores cwlD seemed to have worked judging from the results. A big sigh of relief from our team. Since it has been confirmed that the problem was that insufficient lysozyme stock solution was added, and that the current amount of lysozyme stock solution now works, we proceeded with the treatment for the recovery of spores sleB and cwlJ. The only and main difference is that the spores after treatment were plated out on plates with Kan and Em antibiotic.

We also did the iodine test which came back with relatively good results.

Lastly, we made up a new batch of LB solution for common use.