Team:Newcastle/Labwork/23 July 2009

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Lab Work - 23/07/09

Introduction

Today we will carry out one of the last tasks remaining from our practice lab sessions - precipitating the ethanol from our DNA samples so that gel electrophoresis can be carried out. This can be seen at this link - Introductory Lab Session (23/07/09).

Today also marks the beginning of the real lab work sessions in which the work will be directly involved in implementing our system. The first real task involves growing up E.coli cultures containing the plasmid backbone pMUTIN4, which will allow us in the near future to transfer our chosen BioBricks from the E.coli cells we are working with into Bacillus subtilis.

Another task which we have to complete is to prepare the BioBricks which are needed for our system and are found in the Spring Distributions. These include:

  1. BBa_C0056 – cI repressor (lambda phage)
  2. BBa_B1002 – Terminator sequence
  3. BBa_C0077 – CinR activator
  4. BBa_C0076 – Autoinducer synthetase
  5. BBa_R0077 – Promoter (CinR and HSL regulated, RBS+)



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