Lab Work - 23/07/09

Introduction

Today we will carry out one of the last tasks remaining from our practice lab sessions - precipitating the ethanol from our DNA samples so that gel electrophoresis can be carried out. This can be seen at this link - Introductory Lab Session (23/07/09).

Today also marks the beginning of the real lab work sessions in which the work will be directly involved in implementing our system. The first real task involves growing up E.coli cultures containing the plasmid backbone pMUTIN4, which will allow us in the near future to transfer our chosen BioBricks from the E.coli cells we are working with into Bacillus subtilis.

Another task which we have to complete is to prepare the BioBricks which are needed for our system and are found in the Spring Distributions. These include:

BBa_C0056 – cI repressor (lambda phage)
BBa_B1002 – Terminator sequence
BBa_C0077 – CinR activator
BBa_C0076 – Autoinducer synthetase
BBa_R0077 – Promoter (CinR and HSL regulated, RBS+)

Practical Outline

Precipitate the ethanol from our DNA plasmids (containing the RFP and GFP genes) and analyse them through DNA gel electrophoresis - this is Introductory Lab Session work
Grow up some DH5alpha E.coli cells containing the pMUTIN4 plasmid vector
Hydrate and prepare BioBricks present in Spring Distributions.

20 – 21 June 2009 - Europe workshop (London)
23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
23 October Practice Presentation (Newcastle)
23 October T-shirts are ready
27 October Practice Presentation (Sunderland)
27 October Poster is ready
30 October – 2 November 2009 - Jamboree (Boston)

Team:Newcastle/Labwork/23 July 2009

From 2009.igem.org

Lab Work - 23/07/09

Introduction

Practical Outline