Team:Newcastle/Labwork/24 July 2009

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The introductory lab session work saw the team conduct the first few stages of the ethanol precipitation of the ''GFP'' and ''RFP'' midi-prep samples.
The introductory lab session work saw the team conduct the first few stages of the ethanol precipitation of the ''GFP'' and ''RFP'' midi-prep samples.
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For work done for the introductory lab sessions, [[https://2009.igem.org/Team:Newcastle/IntroductoryLabwork/24_July_2009 please click on this link]]. Meanwhile, as far as real lab work goes, the team needs to transform ''E. coli'' cells with the five BioBricks which were prepared yesterday from the Spring Distributions. The team also need to store away the cultures of ''E. coli'' cells which contain the ''pMUTIN4'' plasmid.
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==Practical Outline==
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By the end of the day the iGEM team need to:
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# Tranform some ''E. coli'' cells (probably Promega ''JM109'' ''E. coli'' cells) with the five BioBricks recently prepared from the Spring Distributions.
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# Prepare the ''E. coli'' cells, which contain the plasmid ''pMUTIN4'', for long term storage.
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Revision as of 19:35, 28 September 2009

Lab session: 24th July

Introduction

The previous lab session has seen the team make a start on the real lab work and also beginning to finish the work involved with the introductory lab sessions.

To begin the real lab work five BioBricks from the Spring Distribution were hydrated and stored in the freezer, namely BioBricks BBA_C0056 (cI in the lambda phage), BBa_B1002 (terminator sequence), BBa_C0077 (CinR activator), BBa_C0076 (autoinducer synthetase) and BBa_R0077 (promoter which is CinR and HSL regulated - RBS+)

The real lab work also involved the team inoculating 2 flasks of 100ml LB with E. coli containing pMUTIN4 and growing the cultures overnight in the 37ºC orbital incubator.

The introductory lab session work saw the team conduct the first few stages of the ethanol precipitation of the GFP and RFP midi-prep samples.

For work done for the introductory lab sessions, [please click on this link]. Meanwhile, as far as real lab work goes, the team needs to transform E. coli cells with the five BioBricks which were prepared yesterday from the Spring Distributions. The team also need to store away the cultures of E. coli cells which contain the pMUTIN4 plasmid.

Practical Outline

By the end of the day the iGEM team need to:

  1. Tranform some E. coli cells (probably Promega JM109 E. coli cells) with the five BioBricks recently prepared from the Spring Distributions.
  2. Prepare the E. coli cells, which contain the plasmid pMUTIN4, for long term storage.



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