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Team:Newcastle/Labwork/3 September 2009
From 2009.igem.org
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Lab Work - 03/09/09
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Today we continued the lab work on our PCR primers. We again attempted to use two pairs of our primers; the ones to amplify the sleB and cwlJ genes from the B. subtilis genome. We prepared the solutions needed, and followed the PCR protocol. We rehydrated our primers, and diluted them accordingly. The TMs were lowered, following yesterday's failed attempt to 50 and 45 degrees. We prepared four lots of solutions, two with genomic DNA and two without (our controls), containing 2 and 3ul of primer. They were loaded into the PCR machine, which ran for around 2 hours 23 minutes.
When we ran the products on a gel.
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- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
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