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Team:Newcastle/Labwork/4 August 2009
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Method B on the other hand, is a slow method which involves stripping of the spore coat layers for improved germination of approximately 10% recovery. | Method B on the other hand, is a slow method which involves stripping of the spore coat layers for improved germination of approximately 10% recovery. | ||
Due to the lack of time, the group decided to go with Method A. | Due to the lack of time, the group decided to go with Method A. | ||
| + | |||
| + | Items needed: | ||
| + | * Lysozyme (not available, ordered) | ||
| + | * 10mM (0.01M) Potassium phosphate (Crystal form available, ordered) | ||
| + | * 50mM (0.05M) KCl (Stock solution of 1M available) | ||
| + | * 1mM (0.001M) MgCl2 (Stock solution of 0.5M available) | ||
| + | * L-alanine (not available, ordered) | ||
Revision as of 17:03, 4 August 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Lab Session 04/08/2009
Sporulation Tuning Team
Prof. Anne Moir from Sheffield University kindly sent us the spores of the genes cwlD and sleB/cwlJ
- HC401 (FrpC2, cwlJ:: Kan, sleB-lacZ Ery R)
- AM1877 (cwlD,Cm r, trp C2)
In the protocol given to us by Prof. Moir, there are two methods (Methods A and B), which can be used to recover the cwlD spores. However, neither give complete restoration of germination.
[Attach Prof. Moir's protocols]
Referring to Prof. Moir's protocols, Method A is a fast method which results in partial germination of approximately 0.1%. Method B on the other hand, is a slow method which involves stripping of the spore coat layers for improved germination of approximately 10% recovery. Due to the lack of time, the group decided to go with Method A.
Items needed:
- Lysozyme (not available, ordered)
- 10mM (0.01M) Potassium phosphate (Crystal form available, ordered)
- 50mM (0.05M) KCl (Stock solution of 1M available)
- 1mM (0.001M) MgCl2 (Stock solution of 0.5M available)
- L-alanine (not available, ordered)
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
