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Team:Newcastle/Metals
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==BioBrick constructs== | ==BioBrick constructs== | ||
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==Lab Work Strategies== | ==Lab Work Strategies== | ||
Revision as of 14:06, 21 October 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Metal Sequester
Introduction
In order to take up and keep the cadmium from the soil efficiently, we needed to think about a way to cross-link the metal ions to intra-cellular proteins which would end up for the most of it in the spore. From our meeting with Prof. Nigel Robinson on the 18th of March 2009, it was evident that the best way of getting cadmium into the spore is to express a metallothionein, which would 'soak up' the cadmium, which in turn would be trapped within the protein in the spore. Using the smtA metallothionein from E.coli and the CotC coat protein as a fusion partner, the metallothionein could in theory be localised to the spore coat as it forms (under control of mother cell specific SigK).
Novelty in this sub-project
Modelling
BioBrick constructs
Lab Work Strategies
Other Presentations and Diagrams
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
