Revision as of 02:46, 14 October 2009

Meetings Calendar

Sporulation Tuning

Introduction

In this section of our project, we hope to control sporulation in our bacterial population, such that we can decide how much of the population becomes spores, and how much continue as vegetative cells. Should the cell sporulate, it would become a ‘metal container’, trapping the sequestered cadmium in its spore.

After the cell sequesters cadmium into its spore, it should not germinate or the sequestered cadmium will be released back into the environment as a result. Therefore, the role of chassis comes into play, where the sleB and cwlJ germination-defective mutants are put into use. More information about this other sub-project of ours can be found here.

Novelty in this sub-project

Instead of letting a cell decide when it wants to sporulate, we hope to influence it's decision. We plan to use kinA as a part of this system.

Modelling

See Team:Newcastle/Modeling/Tuning

BioBrick constructs

A BioBrick which we are designing is to contain an IPTG inducable kinA gene, using pSpac, allowing us to test the theory about kinA in the lab.

Lab Work Strategies

The lab work will mainly be to test our BioBrick using IPTG.

@@ Line 6: / Line 6: @@
 ==Introduction==
-A necessary part of our project is to be able to tune the frequency of sporulation in our system, so that we can make decisions about how much of our bacterial population will become spores, and ultimately “metal containers”.
+In this section of our project, we hope to control sporulation in our bacterial population, such that we can decide how much of the population becomes spores, and how much continue as vegetative cells. Should the cell sporulate, it would become a ‘metal container’, trapping the sequestered cadmium in its spore.
+After the cell sequesters cadmium into its spore, it should not germinate or the sequestered cadmium will be released back into the environment as a result. Therefore, the role of chassis comes into play, where the sleB and cwlJ germination-defective mutants are put into use. More information about this other sub-project of ours can be found [https://2009.igem.org/Team:Newcastle/Chassis here].
 ==Novelty in this sub-project==

Team:Newcastle/SporulationTuning

From 2009.igem.org