Team:UNIPV-Pavia/Notebook/Week1Sep

From 2009.igem.org

(Difference between revisions)
(August, 31st)
(September, 1st)
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== <html><font class="dayw_style">September, 1st</font></html> ==
== <html><font class="dayw_style">September, 1st</font></html> ==
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*We received sequencing results for:
 +
**
 +
**
 +
**
 +
**
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*Glycerol stocks for the 10 B5new2 grown cultures.
 +
 +
*Miniprep for B5new2 (10 samples) and for B8-5.
 +
 +
*Digestion for:
 +
**B5new2 (10 samples) for screening (E-P cut);
 +
**B8-5 for purification from gel (E-P cut);
 +
**R0011 (stored at -20°C) for A17new ligation (S-P cut)
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**E0240 (stored at -20°C) for A17new ligation (X-P cut)
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*Medium-size gel for B5new2(E-P); small-size gel for B8-5(E-P), R0011(S-P) and E0240(X-P).
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*Gel results:
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Revision as of 10:14, 8 September 2009

EthanolPVanimation.gif

December 2008
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March 2009
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30 31
April 2009
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May 2009
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June 2009
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29 30
July 2009
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27 28 29 30 31
August 2009
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September 2009
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October 2009
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November 2009
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30

Week from August 31st, to September 6th, 2009

Previous Week Next Week

August, 31st

  • We inoculated:
    • 10 colonies from B5new2 plate in 4 ml of LB + Kan for screening;
    • B8-5 (8 ul from glycerol stock) in 4 ml of LB + Kan (for purification process);
  • We sent A15-2 purified DNA (stored at -20°C) to BMR Genomics for sequencing.



Experiment with Tecan F200

  • Description
  • Purpose:
  • Materials & Methods
  • Protocol
  • Results

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September, 1st

  • We received sequencing results for:
  • Glycerol stocks for the 10 B5new2 grown cultures.
  • Miniprep for B5new2 (10 samples) and for B8-5.
  • Digestion for:
    • B5new2 (10 samples) for screening (E-P cut);
    • B8-5 for purification from gel (E-P cut);
    • R0011 (stored at -20°C) for A17new ligation (S-P cut)
    • E0240 (stored at -20°C) for A17new ligation (X-P cut)
  • Medium-size gel for B5new2(E-P); small-size gel for B8-5(E-P), R0011(S-P) and E0240(X-P).



  • Gel results:

Top

September, 2nd

Top

September, 3rd

Top

September, 4th<

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