Team:UNIPV-Pavia/Notebook/Week2Jul

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December 2008
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March 2009
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April 2009
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May 2009
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June 2009
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July 2009
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August 2009
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September 2009
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October 2009
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November 2009
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Week from July 6th, to July 12nd, 2009

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July, 6th

  • Digestion for:
R0011(E-X) BOL1(E-S)
  • Gel run/cut and band purification.
  • The purified DNA gave a good result at Nanodrop, but we decided to perform the entire work on BOL1 again because we were not sure that we had extracted a pure band.
  • We stored R0011(E-X) DNA at -20°C.
  • We infected 5 ml of LB + Amp with 8 ul of BOL1 glycerol stock to grow an overnight culture (37°C, 220 rpm).

Preparation of experiment with Tecan F200

  • We picked a colony of B0030 plate (stored at +4°C) and infected 5 ml of LB + Amp.
  • We also infected 5 ml of LB + Amp with 10 ul of A1 and J23100 glycerol stocks.
  • We incubated the inocula overnight at 37°C, 220 rpm.

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July, 7th

  • Miniprep for BOL1 (X2).
  • Digestion for BOL1(E-S)(X2).
  • Gel run/cut/purification.
  • Ligation:
    • A11 = BOL1(E-S) + R0011(E-X) in pSB1A2
  • We incubated the ligation overnight at 16°C.


Preparation of experiment with Tecan F200

  • We diluted 1:100 the overnight cultures of A1, B0030 and J23100.
  • We incubated the diluted cultures for 5 hours (37°C, 220 rpm).

Experiment with Tecan F200


  • Description
  • Purpose:
  • Materials & Methods
  • Protocol
  • Results

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July, 8th

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July, 9th

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July, 10th

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