Team:UNIPV-Pavia/Notebook/Week3Jun

From 2009.igem.org

(Difference between revisions)
m
(Trasformation of Ligations)
Line 19: Line 19:
* 1 ul A6 in Top10 Competent  
* 1 ul A6 in Top10 Competent  
-
Mix 80 ul of SOC with 20 ul X-GAL (40 mg/ml) and plate on LB+Amp plates.
+
Mix 80 ul of SOC with 20 ul X-GAL (40 mg/ml) and infect LB+Amp Agar plates.
* Add 250 ul SOC in 50 ul A5+ Top10  
* Add 250 ul SOC in 50 ul A5+ Top10  
Line 25: Line 25:
Incubated 1 hr 37°C (falcons with shaking 220 rpm) <br>
Incubated 1 hr 37°C (falcons with shaking 220 rpm) <br>
 +
 +
# Infect X-GAL plate with 220 ul A5
== <html><font class="dayw_style">June, 18th</font></html> ==
== <html><font class="dayw_style">June, 18th</font></html> ==

Revision as of 09:10, 18 June 2009

EthanolPVanimation.gif

December 2008
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
March 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
April 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May 2009
M T W T F S S
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June 2009
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August 2009
M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September 2009
M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October 2009
M T W T F S S
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30

Week from June 15th, to June 21st, 2009

June, 15th

June, 16th

June, 17th

Trasformation of Ligations

A5: BBa_Q0040 with BBa_I732017 (RBS-TETR-PTET-RBS-LACZ-TT)
A6: BBa_J23100 with BBa_Q0040 (PCON-RBS-TETR-TT-PTET )

  • 1 ul A5 in Top10 Competent
  • 1 ul A6 in Top10 Competent

Mix 80 ul of SOC with 20 ul X-GAL (40 mg/ml) and infect LB+Amp Agar plates.

  • Add 250 ul SOC in 50 ul A5+ Top10
  • Add 250 ul SOC in 50 ul A6+ Top10

Incubated 1 hr 37°C (falcons with shaking 220 rpm)

  1. Infect X-GAL plate with 220 ul A5

June, 18th

June, 19th

June, 20th

June, 21st

Top