Team:UNIPV-Pavia/Notebook/Week5Jul
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== <html><font class="dayw_style">July, 27th</font></html> == | == <html><font class="dayw_style">July, 27th</font></html> == | ||
- | *Screening for the 11 miniprepped DNA samples for | + | *Screening for the 11 miniprepped DNA samples for B1: digestion S-P for all. |
*Gel results: | *Gel results: | ||
+ | **Samples 1, 3, 5, 6, 8, 10 and 11 showed an extra band for the non ligated plasmid; | ||
+ | **Samples 2, 4, 7 and 9 were pure! we decided to keep B1-7 to perform future ligations. | ||
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+ | *NOTE: we had a pure sample for B1 and a almost-pure sample for B2. Anyway, we decided to perform ligation reactions for these samples and the extra band of B2 will be eliminated during gel cut/purification. Next weeks we will think about purifying B2 itself. | ||
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Revision as of 10:33, 4 August 2009
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Week from July 27th, to August 1st, 2009
Previous Week | Next Week |
July, 27th
- Screening for the 11 miniprepped DNA samples for B1: digestion S-P for all.
- Gel results:
- Samples 1, 3, 5, 6, 8, 10 and 11 showed an extra band for the non ligated plasmid;
- Samples 2, 4, 7 and 9 were pure! we decided to keep B1-7 to perform future ligations.
- NOTE: we had a pure sample for B1 and a almost-pure sample for B2. Anyway, we decided to perform ligation reactions for these samples and the extra band of B2 will be eliminated during gel cut/purification. Next weeks we will think about purifying B2 itself.
July, 28th
July, 29th
July, 30th
July, 31st
Previous Week | Next Week |