Team:UNIPV-Pavia/Notebook/Week5May

From 2009.igem.org

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== <html><font class="dayw_style">May, 25th</font></html> ==
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*iGEM starts now!!!
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*We planned to dedicate the first two weeks to prepare a glycerol stock for every BioBrick of interest for our project. The following weeks will be dedicated to the BioBrick assembly.
*DNA resuspension from iGEM 2009 plates. We resuspended all the high quality constitutive promoter family members from UCBerkeley collection and GFP protein generator:
*DNA resuspension from iGEM 2009 plates. We resuspended all the high quality constitutive promoter family members from UCBerkeley collection and GFP protein generator:

Revision as of 09:39, 20 June 2009

EthanolPVanimation.gif

December 2008
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March 2009
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April 2009
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Week from May 25th, to May 31st, 2009

May, 25th

  • iGEM starts now!!!
  • We planned to dedicate the first two weeks to prepare a glycerol stock for every BioBrick of interest for our project. The following weeks will be dedicated to the BioBrick assembly.
  • DNA resuspension from iGEM 2009 plates. We resuspended all the high quality constitutive promoter family members from UCBerkeley collection and GFP protein generator:
  • Transformation of resuspended DNA (2 ul) in TOP10 E. coli, plated transformed bacteria and incubated the plates overnight at 37°C.

May, 26th

  • All the 15 incubated plates showed colonies!

Moreover, many UCBerkeley promoter family members showed red fluorescent colonies, according to the ranking of promoters' estimated strength (see http://partsregistry.org/Promoters/Catalog/Anderson).

  • We picked one colony from each plate and infected 1 ml of LB + Amp. We incubated the inocula at 37°C, 220 rpm for 5 and 1/2 hours.
  • After 5 hours and 1/2 all the cultures were turbid and bacteria were in their exponential growth phase.
  • Glycerol stocks for the grown cultures.

May, 27th

Experiment with Tecan F200

  • Description
  • Purpose:
  • Materials & Methods
  • Protocol
  • Results

May, 28th

May, 29th

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