Team:UNIPV-Pavia/Notebook/Week5May
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*Transformation of resuspended DNA (2 ul) in TOP10 E. coli, plated transformed bacteria and incubated the plates overnight at 37°C. | *Transformation of resuspended DNA (2 ul) in TOP10 E. coli, plated transformed bacteria and incubated the plates overnight at 37°C. |
Revision as of 09:51, 20 June 2009
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Week from May 25th, to May 31st, 2009
May, 25th
- iGEM starts now!!!
- We planned to dedicate the first two weeks to prepare a glycerol stock for every BioBrick of interest for our project. The following weeks will be dedicated to the BioBrick assembly.
- DNA resuspension from iGEM 2009 plates. We resuspended all the high quality constitutive promoter family members from UCBerkeley collection and GFP protein generator:
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- Transformation of resuspended DNA (2 ul) in TOP10 E. coli, plated transformed bacteria and incubated the plates overnight at 37°C.
May, 26th
- All the 15 incubated plates showed colonies!
Moreover, many UCBerkeley promoter family members showed red fluorescent colonies, according to the ranking of promoters' estimated strength (see http://partsregistry.org/Promoters/Catalog/Anderson).
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- We picked one colony from each plate and infected 1 ml of LB + Amp. We incubated the inocula at 37°C, 220 rpm for 5 and 1/2 hours.
- After 5 hours and 1/2 all the cultures were turbid and bacteria were in their exponential growth phase.
- Glycerol stocks for the grown cultures.
May, 27th
Experiment with Tecan F200
- Description
- Purpose:
- Materials & Methods
- Protocol
- Results
May, 28th
May, 29th
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