Team:Uppsala-Sweden/Notebook

From 2009.igem.org

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{{Uppsala-Sweden Template}}
{{Uppsala-Sweden Template}}
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== '''Notebook''' ==
 
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==Notebook==
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{| align="center"
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|-valign="top"
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=06}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=07}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=08}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=09}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=10}}
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|}
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==Oligonucleotides used by the Uppsala iGEM Team 2009==
==Oligonucleotides used by the Uppsala iGEM Team 2009==
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==Protocols==
==Protocols==
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===PCR Setup===
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====PCR Setup====
[[Media:PCR MM and Primers.xls]]
[[Media:PCR MM and Primers.xls]]
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===Transformation Protocol===
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====Transformation Protocol====
[[Media:Transformation workorder.pdf]]
[[Media:Transformation workorder.pdf]]
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===Growth media for <i>Z. mobilis</i>===
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====Growth media for <i>Z. mobilis</i>====
[[Media:Fullmedium mobilis.xls]]<br>
[[Media:Fullmedium mobilis.xls]]<br>
[[Media:Fullmedium mobilis plus Agar.xls]]
[[Media:Fullmedium mobilis plus Agar.xls]]
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===Growth medium for <i>Synechocystis sp PCC 6803</i>===
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====Growth medium for <i>Synechocystis sp PCC 6803</i>====
[[Media:BG11.pdf]]
[[Media:BG11.pdf]]
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===Growth medium for <i>E. coli</i>===
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====Growth medium for <i>E. coli</i>====
[[Media:LB.pdf]]
[[Media:LB.pdf]]
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===Various===
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====Various====
General Glycerol Stock Protocol:<br>
General Glycerol Stock Protocol:<br>
[[Media:Glycerol Stocks.xls]]
[[Media:Glycerol Stocks.xls]]
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__NOTOC__
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{| align="center"
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|-valign="top"
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=06}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=07}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=08}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=09}}
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|align="center" width="150pt"|{{#calendar: title=Uppsala-Sweden |year=2009 | month=10}}
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|}
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{{Uppsala-Sweden_Footer}}
{{Uppsala-Sweden_Footer}}

Revision as of 17:34, 20 October 2009




Notebook

June
MTWTFSS
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1_June_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/14_June_2009&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/22_June_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/23_June_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/28_June_2009&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/29_June_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/30_June_2009&action=edit 30]
July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1_July_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/2_July_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/8_July_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/9_July_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/12_July_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/13_July_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/14_July_2009&action=edit 14] [http://2009.igem.org/Uppsala-Sweden/15_July_2009 15] [http://2009.igem.org/Uppsala-Sweden/16_July_2009 16] [http://2009.igem.org/Uppsala-Sweden/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/19_July_2009&action=edit 19]
[http://2009.igem.org/Uppsala-Sweden/20_July_2009 20] [http://2009.igem.org/Uppsala-Sweden/21_July_2009 21] [http://2009.igem.org/Uppsala-Sweden/22_July_2009 22] [http://2009.igem.org/Uppsala-Sweden/23_July_2009 23] [http://2009.igem.org/Uppsala-Sweden/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26_July_2009&action=edit 26]
[http://2009.igem.org/Uppsala-Sweden/27_July_2009 27] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/28_July_2009&action=edit 28] [http://2009.igem.org/Uppsala-Sweden/29_July_2009 29] [http://2009.igem.org/Uppsala-Sweden/30_July_2009 30] [http://2009.igem.org/Uppsala-Sweden/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/2_August_2009&action=edit 2]
[http://2009.igem.org/Uppsala-Sweden/3_August_2009 3] [http://2009.igem.org/Uppsala-Sweden/4_August_2009 4] [http://2009.igem.org/Uppsala-Sweden/5_August_2009 5] [http://2009.igem.org/Uppsala-Sweden/6_August_2009 6] [http://2009.igem.org/Uppsala-Sweden/7_August_2009 7] [http://2009.igem.org/Uppsala-Sweden/8_August_2009 8] [http://2009.igem.org/Uppsala-Sweden/9_August_2009 9]
[http://2009.igem.org/Uppsala-Sweden/10_August_2009 10] [http://2009.igem.org/Uppsala-Sweden/11_August_2009 11] [http://2009.igem.org/Uppsala-Sweden/12_August_2009 12] [http://2009.igem.org/Uppsala-Sweden/13_August_2009 13] [http://2009.igem.org/Uppsala-Sweden/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/16_August_2009&action=edit 16]
[http://2009.igem.org/Uppsala-Sweden/17_August_2009 17] [http://2009.igem.org/Uppsala-Sweden/18_August_2009 18] [http://2009.igem.org/Uppsala-Sweden/19_August_2009 19] [http://2009.igem.org/Uppsala-Sweden/20_August_2009 20] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/23_August_2009&action=edit 23]
[http://2009.igem.org/Uppsala-Sweden/24_August_2009 24] [http://2009.igem.org/Uppsala-Sweden/25_August_2009 25] [http://2009.igem.org/Uppsala-Sweden/26_August_2009 26] [http://2009.igem.org/Uppsala-Sweden/27_August_2009 27] [http://2009.igem.org/Uppsala-Sweden/28_August_2009 28] [http://2009.igem.org/Uppsala-Sweden/29_August_2009 29] [http://2009.igem.org/Uppsala-Sweden/30_August_2009 30]
[http://2009.igem.org/Uppsala-Sweden/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Uppsala-Sweden/1_September_2009 1] [http://2009.igem.org/Uppsala-Sweden/2_September_2009 2] [http://2009.igem.org/Uppsala-Sweden/3_September_2009 3] [http://2009.igem.org/Uppsala-Sweden/4_September_2009 4] [http://2009.igem.org/Uppsala-Sweden/5_September_2009 5] [http://2009.igem.org/Uppsala-Sweden/6_September_2009 6]
[http://2009.igem.org/Uppsala-Sweden/7_September_2009 7] [http://2009.igem.org/Uppsala-Sweden/8_September_2009 8] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/9_September_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/10_September_2009&action=edit 10] [http://2009.igem.org/Uppsala-Sweden/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/13_September_2009&action=edit 13]
[http://2009.igem.org/Uppsala-Sweden/14_September_2009 14] [http://2009.igem.org/Uppsala-Sweden/15_September_2009 15] [http://2009.igem.org/Uppsala-Sweden/16_September_2009 16] [http://2009.igem.org/Uppsala-Sweden/17_September_2009 17] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18_September_2009&action=edit 18] [http://2009.igem.org/Uppsala-Sweden/19_September_2009 19] [http://2009.igem.org/Uppsala-Sweden/20_September_2009 20]
[http://2009.igem.org/Uppsala-Sweden/21_September_2009 21] [http://2009.igem.org/Uppsala-Sweden/22_September_2009 22] [http://2009.igem.org/Uppsala-Sweden/23_September_2009 23] [http://2009.igem.org/Uppsala-Sweden/24_September_2009 24] [http://2009.igem.org/Uppsala-Sweden/25_September_2009 25] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/27_September_2009&action=edit 27]
[http://2009.igem.org/Uppsala-Sweden/28_September_2009 28] [http://2009.igem.org/Uppsala-Sweden/29_September_2009 29] [http://2009.igem.org/Uppsala-Sweden/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1_October_2009&action=edit 1] [http://2009.igem.org/Uppsala-Sweden/2_October_2009 2] [http://2009.igem.org/Uppsala-Sweden/3_October_2009 3] [http://2009.igem.org/Uppsala-Sweden/4_October_2009 4]
[http://2009.igem.org/Uppsala-Sweden/5_October_2009 5] [http://2009.igem.org/Uppsala-Sweden/6_October_2009 6] [http://2009.igem.org/Uppsala-Sweden/7_October_2009 7] [http://2009.igem.org/Uppsala-Sweden/8_October_2009 8] [http://2009.igem.org/Uppsala-Sweden/9_October_2009 9] [http://2009.igem.org/Uppsala-Sweden/10_October_2009 10] [http://2009.igem.org/Uppsala-Sweden/11_October_2009 11]
[http://2009.igem.org/Uppsala-Sweden/12_October_2009 12] [http://2009.igem.org/Uppsala-Sweden/13_October_2009 13] [http://2009.igem.org/Uppsala-Sweden/14_October_2009 14] [http://2009.igem.org/Uppsala-Sweden/15_October_2009 15] [http://2009.igem.org/Uppsala-Sweden/16_October_2009 16] [http://2009.igem.org/Uppsala-Sweden/17_October_2009 17] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18_October_2009&action=edit 18]
[http://2009.igem.org/Uppsala-Sweden/19_October_2009 19] [http://2009.igem.org/Uppsala-Sweden/20_October_2009 20] [http://2009.igem.org/Uppsala-Sweden/21_October_2009 21] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/29_October_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/30_October_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/31_October_2009&action=edit 31]


Oligonucleotides used by the Uppsala iGEM Team 2009

All primers we used during the project can be found here: Media:iGEMUppsala09_Primer.xls
Annealing temperatures etc. can be found in the notebook (see below).

Protocols

PCR Setup

Media:PCR MM and Primers.xls

Transformation Protocol

Media:Transformation workorder.pdf

Growth media for Z. mobilis

Media:Fullmedium mobilis.xls
Media:Fullmedium mobilis plus Agar.xls

Growth medium for Synechocystis sp PCC 6803

Media:BG11.pdf

Growth medium for E. coli

Media:LB.pdf

Various

General Glycerol Stock Protocol:
Media:Glycerol Stocks.xls






Bioneer Biolegio Clontech Uppsala Genome Center