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Team:Wisconsin-Madison/Notebook Protocols
From 2009.igem.org
Revision as of 18:26, 20 October 2009 by
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PROTOCOLS
Plasmid Preparation by Alkaline Lysis
Plasmid Preparation by Boiling Lysis
Restriction Enzyme Mapping
Transformation of Plasmids into Competent Cells
Preparation of Competent Cells
Overview of Cloning Protocol
** GREAT for New Teams! **
Polymerase Chain Reaction (PCR)
Ligation
Digestion - Cloning
Loading Gel Protocol
Sequencing Reaction
Colony Polymerase Chain Reaction (PCR)
Genomic DNA Extraction
Safety