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Uppsala-Sweden/10 October 2009
From 2009.igem.org
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(New page: {{Uppsala-Sweden Template}} ==Plasmid prep== A plasmid prep was performed for PRPRYT 3 & 4 on pSB1AK3. ==Triparental mating== BG-11 plates were made without antibiotics and without CuS...) |
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BG-11 plates were made without antibiotics and without CuSO4 to have the pPetE promoter as silent as possible during triparental mating. | BG-11 plates were made without antibiotics and without CuSO4 to have the pPetE promoter as silent as possible during triparental mating. | ||
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| + | ==Evaluate constructs in ''e.coli''== | ||
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| + | The constructs were assembled with the lac promoter (R0011) to make it possible to evaluate them in ''e.coli'', transformed into M15 carrying the lac repressor making it possible for us to induce with IPTG. | ||
{{Uppsala-Sweden_Footer}} | {{Uppsala-Sweden_Footer}} | ||
Latest revision as of 16:10, 21 October 2009
Plasmid prep
A plasmid prep was performed for PRPRYT 3 & 4 on pSB1AK3.
Triparental mating
BG-11 plates were made without antibiotics and without CuSO4 to have the pPetE promoter as silent as possible during triparental mating.
Evaluate constructs in e.coli
The constructs were assembled with the lac promoter (R0011) to make it possible to evaluate them in e.coli, transformed into M15 carrying the lac repressor making it possible for us to induce with IPTG.
