From 2009.igem.org
| August
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| M | T | W | T | F | S | S
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| [http://2009.igem.org/User:DavidC/1_August_2009 1]
| [http://2009.igem.org/User:DavidC/2_August_2009 2]
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| [http://2009.igem.org/User:DavidC/3_August_2009 3]
| [http://2009.igem.org/User:DavidC/4_August_2009 4]
| [http://2009.igem.org/User:DavidC/5_August_2009 5]
| [http://2009.igem.org/User:DavidC/6_August_2009 6]
| [http://2009.igem.org/User:DavidC/7_August_2009 7]
| [http://2009.igem.org/User:DavidC/8_August_2009 8]
| [http://2009.igem.org/User:DavidC/9_August_2009 9]
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| [http://2009.igem.org/User:DavidC/10_August_2009 10]
| [http://2009.igem.org/User:DavidC/11_August_2009 11]
| [http://2009.igem.org/User:DavidC/12_August_2009 12]
| [http://2009.igem.org/User:DavidC/13_August_2009 13]
| [http://2009.igem.org/User:DavidC/14_August_2009 14]
| [http://2009.igem.org/User:DavidC/15_August_2009 15]
| [http://2009.igem.org/User:DavidC/16_August_2009 16]
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| [http://2009.igem.org/User:DavidC/17_August_2009 17]
| [http://2009.igem.org/User:DavidC/18_August_2009 18]
| [http://2009.igem.org/User:DavidC/19_August_2009 19]
| [http://2009.igem.org/User:DavidC/20_August_2009 20]
| [http://2009.igem.org/User:DavidC/21_August_2009 21]
| [http://2009.igem.org/User:DavidC/22_August_2009 22]
| [http://2009.igem.org/User:DavidC/23_August_2009 23]
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| [http://2009.igem.org/User:DavidC/24_August_2009 24]
| [http://2009.igem.org/User:DavidC/25_August_2009 25]
| [http://2009.igem.org/User:DavidC/26_August_2009 26]
| [http://2009.igem.org/User:DavidC/27_August_2009 27]
| [http://2009.igem.org/User:DavidC/28_August_2009 28]
| [http://2009.igem.org/User:DavidC/29_August_2009 29]
| [http://2009.igem.org/User:DavidC/30_August_2009 30]
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| [http://2009.igem.org/User:DavidC/31_August_2009 31]
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| September
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| M | T | W | T | F | S | S
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| [http://2009.igem.org/User:DavidC/1_September_2009 1]
| [http://2009.igem.org/User:DavidC/2_September_2009 2]
| [http://2009.igem.org/User:DavidC/3_September_2009 3]
| [http://2009.igem.org/User:DavidC/4_September_2009 4]
| [http://2009.igem.org/User:DavidC/5_September_2009 5]
| [http://2009.igem.org/User:DavidC/6_September_2009 6]
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| [http://2009.igem.org/User:DavidC/7_September_2009 7]
| [http://2009.igem.org/User:DavidC/8_September_2009 8]
| [http://2009.igem.org/User:DavidC/9_September_2009 9]
| [http://2009.igem.org/User:DavidC/10_September_2009 10]
| [http://2009.igem.org/User:DavidC/11_September_2009 11]
| [http://2009.igem.org/User:DavidC/12_September_2009 12]
| [http://2009.igem.org/User:DavidC/13_September_2009 13]
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| [http://2009.igem.org/User:DavidC/14_September_2009 14]
| [http://2009.igem.org/User:DavidC/15_September_2009 15]
| [http://2009.igem.org/User:DavidC/16_September_2009 16]
| [http://2009.igem.org/User:DavidC/17_September_2009 17]
| [http://2009.igem.org/User:DavidC/18_September_2009 18]
| [http://2009.igem.org/User:DavidC/19_September_2009 19]
| [http://2009.igem.org/User:DavidC/20_September_2009 20]
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| [http://2009.igem.org/User:DavidC/21_September_2009 21]
| [http://2009.igem.org/User:DavidC/22_September_2009 22]
| [http://2009.igem.org/User:DavidC/23_September_2009 23]
| [http://2009.igem.org/User:DavidC/24_September_2009 24]
| [http://2009.igem.org/User:DavidC/25_September_2009 25]
| [http://2009.igem.org/User:DavidC/26_September_2009 26]
| [http://2009.igem.org/User:DavidC/27_September_2009 27]
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| [http://2009.igem.org/User:DavidC/28_September_2009 28]
| [http://2009.igem.org/User:DavidC/29_September_2009 29]
| [http://2009.igem.org/User:DavidC/30_September_2009 30]
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| October
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| M | T | W | T | F | S | S
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| [http://2009.igem.org/User:DavidC/1_October_2009 1]
| [http://2009.igem.org/User:DavidC/2_October_2009 2]
| [http://2009.igem.org/User:DavidC/3_October_2009 3]
| [http://2009.igem.org/User:DavidC/4_October_2009 4]
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| [http://2009.igem.org/User:DavidC/5_October_2009 5]
| [http://2009.igem.org/User:DavidC/6_October_2009 6]
| [http://2009.igem.org/User:DavidC/7_October_2009 7]
| [http://2009.igem.org/User:DavidC/8_October_2009 8]
| [http://2009.igem.org/User:DavidC/9_October_2009 9]
| [http://2009.igem.org/User:DavidC/10_October_2009 10]
| [http://2009.igem.org/User:DavidC/11_October_2009 11]
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| [http://2009.igem.org/User:DavidC/12_October_2009 12]
| [http://2009.igem.org/User:DavidC/13_October_2009 13]
| [http://2009.igem.org/User:DavidC/14_October_2009 14]
| [http://2009.igem.org/User:DavidC/15_October_2009 15]
| [http://2009.igem.org/User:DavidC/16_October_2009 16]
| [http://2009.igem.org/User:DavidC/17_October_2009 17]
| [http://2009.igem.org/User:DavidC/18_October_2009 18]
|
| [http://2009.igem.org/User:DavidC/19_October_2009 19]
| [http://2009.igem.org/User:DavidC/20_October_2009 20]
| [http://2009.igem.org/User:DavidC/21_October_2009 21]
| [http://2009.igem.org/User:DavidC/22_October_2009 22]
| [http://2009.igem.org/User:DavidC/23_October_2009 23]
| [http://2009.igem.org/User:DavidC/24_October_2009 24]
| [http://2009.igem.org/User:DavidC/25_October_2009 25]
|
| [http://2009.igem.org/User:DavidC/26_October_2009 26]
| [http://2009.igem.org/User:DavidC/27_October_2009 27]
| [http://2009.igem.org/User:DavidC/28_October_2009 28]
| [http://2009.igem.org/User:DavidC/29_October_2009 29]
| [http://2009.igem.org/User:DavidC/30_October_2009 30]
| [http://2009.igem.org/User:DavidC/31_October_2009 31]
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Sunday the 20th
Restriction digest
P1003 (= 2,5µg/µL) restriction digest by EcoRI and SpeI:
DNA (10µg final) = 4µL
Buffer H (TAKARA) =2µL
H20 = 12µL
EcoR1 (TAKARA) = 1µL
Spe 1 (TAKARA) = 1µL
>
Incubation 1h at 37°C.
Calf intestine phosphatase (CIP) processing:
Restriction digest mix = 20µL
CIP Buffer (TAKARA) = 5µL
H20 = 24µL
CIP (TAKARA) = 1µL
1h at 37°C
DNA electrophoresis
85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.
First: Purification of P1003 fragment:
Second: Quantization of P1003; C0012 and B0014:
DNA purification
Kit Qiagen “gel extraction kit”, final volume = 50µL.
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