August/11 October 2009

From 2009.igem.org

1.colony check

sample        no. of colony
chiba 2             +
chiba 3             ++
chiba 4             +
 31                   +++
 58                    5
 62                    3 
 73                     0
 76                    >20
 77                     7  
 78                    >30

2. min prep

sample                conc.
 1-23L                   20 ng/uL
 1-23L                   113
  22                        196
  47                        167
  40                        130
  25                        164
  31                        31   
  X4                       265
  X4                       269
  59                       142
  70                       325
  72                       281

3.diagtion

K204079

Vector		Insert
1-23L	3	chiba 1	4
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	11	dH₂O	11
total	20uL	total	20uL

K204080

Vector		Insert
1-23L	3	chiba 5	4
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	11	dH₂O	11
total	20uL	total	20uL

K204081

Vector		Insert
1-23L	2	chiba 6	5
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	12	dH₂O	9
total	20uL	total	20uL

K204082

Vector		Insert
1-23L	2	chiba 7	5
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	12	dH₂O	9
total	20uL	total	20uL

K204083

Vector		Insert
1-23L	3	chiba 8	4
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	11	dH₂O	10
total	20uL	total	20uL

K204084

Vector		Insert
1-23L	2	chiba 9	5
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	12	dH₂O	9
total	20uL	total	20uL

K204067

Vector		Insert
1-23L	6	2-16L	10
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	10	dH₂O	4
total	20uL	total	20uL

K204068

Vector		Insert
1-23L	10	1-19B	5
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	4	dH₂O	4
total	20uL	total	20uL

K204071

Vector		Insert
1-8K	2	5	4
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	12	dH₂O	10
total	20uL	total	20uL

K204075

Vector		Insert
1-1K	10	1-8K	2
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	4	dH₂O	12
total	20uL	total	20uL

↓
37 degree , 2hr
gel cut & purification
ligation

4. min prep

sample                conc.
plac CFP               314 ng/uL
ptet CFP               179
plac mOrange      45
ptet mOrange      170

back to NOTES