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August/30 August 2009
From 2009.igem.org
1. ligation rapid check
UP : 13-A,B,C , 14-A,B,C , 19-A,B,C , vector ,ladder DOWN : 12-A,B,C , 15-A,B,C , 16-A,B,C , vector , ladder
No.13 (RBS+rhlI),K204013
13-A,C : OK
No.14 (RBS+LasI),K204014
14 -A,C : OK
No.19 (RBS+RFP(orange?)),K204019
19-A,B : OK
No.12 (RBS+cinR+terminater),K204012
12-B : OK ?
No.15 (RBS+cinI+terminater), K204015
15-A,B,C : OK?
No.16 (RBS+LuxI+terminater) , K204016
16-A,B,C ok?
2.digestion and ligation
digestion with restriction enzyme
K204020
| Vector | Insert | ||
|---|---|---|---|
| p(tetR)(1-6I) | 5 | YFP(1-8I) | 5 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 Buffer | 2 | No.2 | 2 |
| dH2O | 11 | dH2O | 4 |
| total | 20uL | total | 20uL |
K204021
| Vector | Insert | ||
|---|---|---|---|
| p(tetR)(1-6I) | 5 | CFP(1-8K) | 5 |
| SpeI | 1 | XbaI | 1 |
| PstI | 1 | PstI | 1 |
| No.2 Buffer | 2 | No.2 | 2 |
| dH2O | 11 | dH2O | 11 |
| total | 20uL | total | 20uL |
3.12-B , 13-A , 14-A , 15-A , 16-A , 19-A were inoculated into growth culture solution.
Miniprep should be conducted on the resulting cell cultures tomorrow.
