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Lab Aug 5 2009
From 2009.igem.org
Miniprep 4 previous parts (B0015 Amp/Kan, J06504 Amp, R0051 Amp, B0034 Amp) from overnight cultures: Qiagen Miniprep Protocol
gel 4 parts: Agarose Gel Electrophoresis Protocol
visualized the gel, pasted into Ky's notebook
nanodropped the RFP generator following the miniprep, and using TE as the elution buffer to zero the nanodrop
21 ng/ul
260/280 ratio of 1.54
260 to 230 ratio of 1.81
R0051 - lambda cI promoter, we re-ran the transformation protocol with more of the rehydrated pklasmid
Rehydrated and Transformed the following parts:
I0050 - arabinose promoter on a Kan plate, add IPTG to broth culture to induce high copy
R0010 - lactose promoter
J23032 - ribolock RBS
J23066 - ribokey
C0051 - lambda cI with the LVA tag
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