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Lab July 3 2009
From 2009.igem.org
July 3, 2009
500 mL of 100 mM EDTA at pH 7.0
14.61 g EDTA in ~250mL water
pH to 7.0 and top up to 500 mL
1L TE buffer @ 10mM Tris pH 8.0 and 1mM EDTA
10 mL Tris 8.0 stock, 10 mL EDTA stock, top up to 1L
1L P1 buffer @ 50 mM Tris pH 8.0 and 10 mM EDTA
50 mL Tris 8.0 stock
100 mL 100 mM EDTA stock
(RNaseA to be added at usage time)
1L PE
10mM Tris-HCL pH 7.5, 80% ethanol
842 mL 95% ethanol (equiv to 800 ml of 100% ethanol)
10ml Tris 7.5 1M stock
top up to 1L with distilled water
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