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User contributions
From 2009.igem.org
- 03:04, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/recipes (New page: {{Team:Waterloo/NavBar}} __TOC__ ==Making LB Media== <b>Materials </b><br> 500 mL media bottle or rack of test tubes <br> 1 L beaker <br> Magnetic stir bar <br> Tryptone <br> Yeast extrac...) (top)
- 03:03, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/Protocols/Competent Cells (New page: {{Team:Waterloo/NavBar}} <big><b><u>Recipe for Competent Cells </u></b></big> <br> <b>Materials </b><br> Frozen stock of DH5 alpha <br> 250mL Erlenmeyer Flask (w/ caps) <br> LB tubes <...) (top)
- 03:00, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/Protocols/Transformation (New page: {{Team:Waterloo/NavBar}} <b><u><big>Transformation</big></u></b><br> <b>Before you start </b><br> Cells lose their competence once thawed, so be sure to:<br> a) keep them on ice at all t...) (top)
- 02:59, 22 October 2009 (diff | hist) Team:Waterloo/Notebook/Protocols/Inoculation (top)
- 02:58, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/Protocols/Inoculation (New page: {{Team:Waterloo/NavBar}} <big><b><u>Inoculation Protocol </u></b></big><br> <b>Materials </b><br> DNA Samples <br> Ampicillin (2µL/sample)<br> Kanamycin (2.5µL/sample)<br> Liquid LB Me...)
- 02:57, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation (New page: {{Team:Waterloo/NavBar}} <b><u><big>Glycerol Stock Preparation </big></u></b><br> <b>Before you start </b><br> - It is important to use aseptic technique when carrying out the procedure...) (top)
- 02:56, 22 October 2009 (diff | hist) Team:Waterloo/Notebook/Protocols/Ligation (top)
- 02:55, 22 October 2009 (diff | hist) Team:Waterloo/Notebook/Protocols/Ligation
- 02:55, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/Protocols/Ligation (New page: {{Team:Waterloo/NavBar}} <big><b><u>Ligation (10µL recipe with 5 insert : 1 vector ratio)</u></b></big> <br> <b>Materials </b><br> MQ water <br> Ligase buffer<br> Ligase <br> Eppendorf...)
- 02:54, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/Protocols/Gel Electrophoresis (New page: {{Team:Waterloo/NavBar}} <b><u><big>Gel Electrophoresis</big></u></b><br> <b>Materials </b><br> Agarose <br> 1X TAE buffer <br> Gel Red<br> 1:10 diluted 1kb DNA ladder<br> Loading Dye<...) (top)
- 02:53, 22 October 2009 (diff | hist) Team:Waterloo/Notebook/Protocols/DNA Extraction (top)
- 02:51, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/Protocols/DNA Extraction (New page: {{Team:Waterloo/NavBar}} <b><u><big>DNA Extraction </big></u></b><br> <b>Materials </b><br> All required materials provided in the Fermentas DNA Extraction Kit (K0513)<br> <b>Instructio...)
- 02:47, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/Protocols/DNA Digestion (New page: {{Team:Waterloo/NavBar}} <b><u><big>Digestion of Plasmid DNA </big></u></b><br> <b>Before you start </b><br> - Know what is the total digestion volume mix. From that you can determine th...) (top)
- 02:44, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/Protocols/Colony PCR (New page: {{Team:Waterloo/NavBar}} <b><u><big>Colony PCR </big></u></b><br> <b>Materials</b><br> - 20 µL of Master Mix<br> <u>Calculations for Master Mix</u><br> ___ number of colonies (and con...) (top)
- 02:43, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/Protocols/Miniprep (New page: {{Team:Waterloo/NavBar}} <b><u><big>1-2-3 Miniprep (Resuspension, Lysis, Neutralization)</big></u></b><br> <b>Before you start</b><br> Prepare solutions 1, 2, and 3. Only solution 2 need...) (top)
- 02:24, 22 October 2009 (diff | hist) Team:Waterloo/Notebook/protocols (top)
- 02:22, 22 October 2009 (diff | hist) Team:Waterloo/Notebook/protocols
- 02:21, 22 October 2009 (diff | hist) N Team:Waterloo/Notebook/protocols (New page: * 1-2-3- Mini-prep * Colony PCR * [[Team:Waterloo/Notebook/Protocols/DNA Digestion|DNA Digesti...)
- 02:19, 22 October 2009 (diff | hist) Team:Waterloo/Notebook (top)
- 02:05, 22 October 2009 (diff | hist) Team:Waterloo/Team (→Who we are) (top)
- 22:37, 20 October 2009 (diff | hist) Team:Waterloo/Notebook (→Oligos)
- 22:34, 20 October 2009 (diff | hist) Team:Waterloo/Project (→Landing Pad Strain)
- 22:33, 20 October 2009 (diff | hist) Team:Waterloo/Project (→Landing Pad Strain)
- 22:33, 20 October 2009 (diff | hist) Team:Waterloo/Project (→Landing Pad Strain)
- 22:19, 20 October 2009 (diff | hist) Team:Waterloo/Project (→Landing Pad Strain)
- 22:18, 20 October 2009 (diff | hist) N File:PFB9009 Map.png (top)
- 22:14, 20 October 2009 (diff | hist) Team:Waterloo/Project (→Landing Pad Strain)
- 22:10, 20 October 2009 (diff | hist) Team:Waterloo/Project (→Results)
- 02:37, 17 October 2009 (diff | hist) Team:Waterloo/Project (→Landing Pad Strain)
- 02:35, 17 October 2009 (diff | hist) Team:Waterloo/Project (→Landing Pad Strain)
- 00:38, 17 October 2009 (diff | hist) Team:Waterloo/Team (→Undergraduate Students)
- 00:36, 17 October 2009 (diff | hist) N File:Tim Picture2.jpg (top)
- 00:23, 17 October 2009 (diff | hist) N File:Tim Picture.jpg (top)
