Team:Imperial College London/Wetlab/Results/OD calibration

From 2009.igem.org


We have calibrated the absorbance at 600nm (O.D.600) with the colony forming units. This allows us to approximate our absorbance data into a cell count number. We can now normalise fluorescence data to obtain the fluorescence produced per cell.

We performed the experiment and further reruns with 2-3 repeats per OD value and for 2 different dilutions.

Mr. Gene   Geneart   Clontech   Giant Microbes

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