Team:KULeuven/13 July 2009
From 2009.igem.org
Contents |
Project progress
A & B (comparator), ideas
Key/lock system, see Riboregulators (Berkeley 2006)
Odor synthesis/sensor
- Vanillin synthesis: DNA not yet complete/in library [1]
- Combination of 5 genes; 3 are available
- Edinburgh Vanilla production (Synthesis pathway by Edinburgh igem 2007)
- Vanillin production using metabolically engineered Escherichia coli under non-growing conditions, Ferrulic accid --> Vanillin, 5k bp
- Combination of 5 genes; 3 are available
Light sensor
- YcgF Gene, natural available in 3 E. Coli strains. Can use the promotor of YcgF gene for expression
- Probable strain K12
- Primer of YcgF Promotor:
- AACAATCCAGGGTAATGGGTGAGGCGAGAGTAAGACGGTAACAGACATATCTTCTTG TGTCTTTCTTTTAATACCAAAACATAACCGTTTCTTTACATTGATAAAAAATGGAAAAAG TTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTT TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATT CTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG (bron: [www.ncbi.nlm.nih.gov])
Odourless strain of E.coli
- MIT 2006 used an indoline deficient strain of E.coli: YYC912.
- Knockout of TNAa5- gene (tryptophanase) in various strains and selected the least 'smelly.'
To do
- Blue light receptor
- Decide on E.Coli strain
- Try with K12
- Vector in neural scent (indole knockout)
- Decide on primers (!Pre + suffix!)
- PCR of promotor
- Couple promotor to GFP --> Standard iGEM plasmid
- Test!
- Obtain regulation site
- Decide on E.Coli strain
- Vanillin synthesis
- Send mail to University of Tuscia
- Presentations
- Order receptor biobrick
- Trg - Env2
- PBP
Drylab
Wiki
- Create Ideas page
- Some idiot replaced the example logo Image:Example_logo.png with his own logo, so all references to it were removed from our wiki
Progress of parts
[edit] Blue Light Receptor
- YcgF Gene, naturally available in 3 E. Coli strains. Can use the promotor of Ycgf gene for expression
- Probable strain K12
- Promotor voor YcgF gene:
- AACAATCCAGGGTAATGGGTGAGGCGAGAGTAAGACGGTAACAGACATATCTTCTTG TGTCTTTCTTTTAATACCAAAACATAACCGTTTCTTTACATTGATAAAAAATGGAAAAAG TTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTT TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATT CTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG
(source: [2])
- AACAATCCAGGGTAATGGGTGAGGCGAGAGTAAGACGGTAACAGACATATCTTCTTG TGTCTTTCTTTTAATACCAAAACATAACCGTTTCTTTACATTGATAAAAAATGGAAAAAG TTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTT TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATT CTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG
[edit] Vanillin Production
- Vanillin synthesis: DNA not yet complete/in library [3]
- Combination of 5 genes; 3 are available
- Edinburgh Vanilla production (Pathway synthesis, Edinburgh iGEM 2007)
- Vanillin production using metabolically engineered Escherichia coli under non-growing conditions, Ferulic accid --> Vanillin, 5k bp
- Combination of 5 genes; 3 are available