Mix: | 50μl
|
template | 1μl
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forward primer - | 1μl
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reverse primer - | 1μl
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dNTP | 2μl
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10x buffer | 5μl
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taq | 0.25μl
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ddH20 | 39.75 μl
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Protocol:
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1. | 94oC: 60
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2. 30 cycles | 94oC: 15
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55oC: 20
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72oC: 150
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3. | 72oC: 300
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| 2% agarose, 90V, 30min
Descr | Win length | Fail length
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0: marker 1kb+100bp | |
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1: gp120 full length | 2621bp |
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2: gp120 a-b | 2539bp |
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3: H1N1-HA full length | 1742bp |
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4: H5N1-HA full length | 1742bp |
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5: H1N1-NA full length | 1460bp |
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6: H5N1-NA full length | 1391bp |
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7: H5N1-NA a-b | 1283bp |
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8: Positive Control BBa_E0240 | 1114bp (876+238) | 238bp
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9: H5N1-HA b-c | 911 |
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10:H1N1-NA c-d | 606bp |
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11:H1N1-NA d-e | 495bp |
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12:Positive Control pLac | 437bp |
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13:H5N1-HA d-e | 378bp |
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14:H1N1-NA b-c | 315bp |
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15:H5N1-HA a-b | 285bp |
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16:marker 100bp | |
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