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From 2009.igem.org

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August 11, 2009

1. Digested 4, 5, Enc, C(E+P), C(X+S)
   • BB4 did not digest
   • 5, C is stored in fridge
2. Calvin suggested increasing the concentration of enzyme and increase time of digestion due to thawing enzymes over weekend in fridge
3. Miniprepped 1+2(1) with new kit, got great yield ~100ng/ul
4. Gel extracted C (X+S) after it was CIPed
5. Overnight ligation of Enc+C in PCR machine
6. Calvin gave us 2 vials of RbCl cells in -80C
   • one time use only
   • thaw and add DNA

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