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From 2009.igem.org

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August 14, 2009

1. Miniprepped 5, 7, C with normal protocol of BioBasics Kit
   • Results were low ~30ng/ul
2. Digested C and CIPed it
3. Ran 4 digest and plasmid samples of 5, 7, C and the CIPed C plasmid samples of 5, 7, C and the CIPed C plasmid
4. Gel extracted CIPed C plasmid
5. Ligated 4+5 and Enc into C plasmid and plated

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