Week from September 14th, to September 20th, 2009

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September, 14th

• Cloning
• Wiki updating

September, 15th

• Fermentation experiment setup
• Wiki updating

September, 16th

• Fermentation experiment
• Wiki updating

September, 17th

Experiment with Tecan F200

• Download Protocol
• Download Protocol

pH sensor

• Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
  • RBS33
  • A2
  • K116002 (from now on it will be indicated only as pNhaA)
• Overnight incubation of these three cultures at 37°C, 220 rpm.

September, 18th

pH sensor

1st experiment

• We put 50 ul into 5 ml LBK + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
• OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
• We diluted:
  • RBS into:
    • LBK + Amp pH 5,5
    • LBK + Amp pH 6,6
    • LBK + Amp pH 7,5
    • LBK + Amp pH 8,5
  • pNhaA into:
    • LBK + Amp pH 5,5
    • LBK + Amp pH 6,6
    • LBK + Amp pH 7,5
    • LBK + Amp pH 8,5
  • A2 into:
    • LBK + Amp pH 5,5
    • LBK + Amp pH 6,6
    • LBK + Amp pH 7,5
    • LBK + Amp pH 8,5
• We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.

September, 19th

• Wiki updating

September, 20th

• Cloning
• Wiki updating

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