| Location | Strain # | Name | Chassis/Strain | Plasmid Backbone | Registry Part | Antibiotic Resistance | Description | Reference Date
|
| iGEM strain box 1
| 1
| rTT + PocI
| DH5α
| pMA (GeneArt)
| [http://partsregistry.org/Part:BBa_K093003 BBa_K093003]
| Amp100
| Synthesized. Contains BioBrick prefix and suffix. Reverse orientation transcriptional terminator R0015 with λ Pr promotor.
| 2008:09:09
|
| iGEM strain box 1
| 2
| rPlacTT
| DH5α
| pMA (GeneArt)
| [http://partsregistry.org/Part:BBa_K093004 BBa_K093004]
| Amp100
| Synthesized. Contains BioBrick prefix and suffix. Reverse [http://partsregistry.org/Part:BBa_R0011 BBa_R0011] and transcriptional terminator [http://partsregistry.org/Part:BBa_B0015 BBa_B0015].
| 2008:09:09
|
| iGEM strain box 1
| 3
| PmeI
| DH5α
| pMA (GeneArt)
| [http://partsregistry.org/Part:BBa_K093002 BBa_K093002]
| Amp100
| Synthesized ORF. Contains BioBrick prefix and suffix.
| 2008:09:09
|
| iGEM strain box 1
| 4
| rfp'-14
| DH5α
| pSB1A2
| [http://partsregistry.org/Part:BBa_K093005 BBa_K093005]
| Amp100
| RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) colony 14.
| 2008:09:09
|
| iGEM strain box 1
| 5
| rfp-2
| DH5α
| pSB1AK3
|
| Amp100, Kan25
| RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) colony 2. Might need testing
| 2008:09:09
|
| iGEM strain box 1
| 6
| Pconst-rfp-1
| DH5α
| pSB1A2
| [http://partsregistry.org/Part:BBa_K093012 BBa_K093012]
| Amp100
| RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) under a constitutive promoter ([http://partsregistry.org/Part:BBa_J23118 BBa_J23118]). Might need testing.
| 2008:09:09
|
| iGEM strain box 1
| 7
| pRecA-3
| DH5α
| psb2k3
| [http://partsregistry.org/Part:BBa_K093000 BBa_K093000]
| Kan25
| Engineered RecA promoter with lexA consensus sequence.
| 2008:10:17
|
| iGEM strain box 1
| 8
| Pconst-rfp' 1
| DH5α
| psb1a2
|
| Amp100
| RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) under a constitutive promoter ([http://partsregistry.org/Part:BBa_J23118 BBa_J23118])
| 2008:10:17
|
| iGEM strain box 1
| 9
| c0051'
| DH5α
| psb1a2
| [http://partsregistry.org/Part:BBa_K093006 BBa_K093006]
| Amp100
| [http://partsregistry.org/Part:BBa_C0051 BBa_C0051] Repressor for Lambda Pr promoter with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]). Never got sequencing results of prefix. Rest was good
| 2008:10:17
|
| iGEM strain box 1
| 10
| PmeI - 14
| DH5α
| psb2k3
| [http://partsregistry.org/Part:BBa_K093002 BBa_K093002]
| Kan25
| PmeI cloned into BioBrick vector
| 2008:10:17
|
| iGEM strain box 1
| 11
| PrecA RFP'
| DH5α
| pSB2K3
| [http://partsregistry.org/Part:BBa_K093009 BBa_K093009]
| Kan25
| RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) under the control of [http://partsregistry.org/Part:BBa_K093000 BBa_K093000] promoter
| 2008:10:17
|
| iGEM strain box 1
| 12
| PmeI'
| DH5α
| pSB1A2
| [http://partsregistry.org/Part:BBa_K093013 BBa_K093013]
| Amp100
| PmeI ([http://partsregistry.org/Part:BBa_K093002 BBa_K093002]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034])
| 2008:11:04
|
| iGEM strain box 1
| 13
| Pr RFP'
| DH5α
| psb1a2
| [http://partsregistry.org/Part:BBa_K093010 BBa_K093010]
| Amp100
| RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) under Lamda Pr promoter (R0051)
| 2008:11:04
|
| iGEM strain box 1
| 14
| T7 gene 6
| DH5α
| pSB1A2
| [http://partsregistry.org/Part:BBa_K093001 BBa_K093001]
| Amp100
| good one, correct stop codon taataa
| 2009:02:13
|
| iGEM strain box 1
| 15
| PkNG101
| DH5α λ pir
|
|
| Strep100
| Only replicates in λ pir lysogen (r6k origin of replication) http://bccm.belspo.be/db/lmbp_plasmid_details.php?NM=pKNG101
| 2009:02:xx
|
| iGEM strain box 1
| 16
| H1-4 pJET
| DH5α
| pJET
|
| Amp100
| E. coli melA genomic DNA for homologous recombination (“left sequence”/H1) and attP site downstream
| 2009:03:18
|
| iGEM strain box 1
| 17
| CI pJET
| DH5α
| pJET
|
| Amp100
| CI repressor for lamda Pr promoter. [http://partsregistry.org/Part:BBa_C0051 BBa_C0051] with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) in pJET (made by crossover PCR)
| 2009:03:xx
|
| iGEM strain box 1
| 18
| pFB9002
| DH5α lamda pir
| pfb9001
|
| Strep 100
| E. coli melA genomic DNA for homologous recombination (“left sequence”/H1) and attP site downstream
| 2009:03:xx
|
| iGEM strain box 1
| 19
| pJET H2
| DH5α
| pJET
|
| Amp100
| E. coli melA genomic DNA for homologous recombination (“right sequence”/H2) and attP site upstream
| 2009:04:09
|
| iGEM strain box 1
| 20
| Pfb9003
| DH5α lamda pir
| pfb9001
|
| Strep100
| E. coli melA genomic DNA for homologous recombination (“right sequence”/H2) and attP site upstream
| 2009:04:23
|
| iGEM strain box 1
| 21
| pfb9004
| DH5α lamda pir
| pfb9001
|
| Strep100
| Contains two attP sites flanked by E. coli melA genomic DNA for homologous recombination (H1 and H2)
| 2009:04:23
|
| iGEM strain box 1
| 22
| pJET rfp-1
| DH5α
| pJET
|
| Amp100
| insert: Pconst ([http://partsregistry.org/Part:BBa_J23118 BBa_J23118]) rfp ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) EcoRV fragment for propagating pJET
| 2009:04:24
|
| iGEM strain box 1
| 79
| pJET rfp-2
| DH5α
| pJET
|
| Amp100
| insert: Pconst rfp EcoRV fragment
| 2009:04:24
|
| iGEM strain box 1
| 81
| pJET rfp-3
| DH5α
| pJET
|
| Amp100
| insert: Pconst rfp EcoRV fragment
| 2009:04:24
|
| iGEM strain box 1
| 23
| RBS
| DH5α
| pSB1A2?
| B0034
| Amp100
| Ribosome binding site (Elowitz)
|
|
| iGEM strain box 1
| 24
| Pconst55%
| DH5α
| [http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
| [http://partsregistry.org/Part:BBa_J23118 BBa_J23118]
| Amp100
| Constitutive promoter. Expression level is 55% in comparison with the highest expression levels from this Pconst family (ex. wildtype promoter)
|
|
| iGEM strain box 1
| 25
| TT
| DH5α
| pSB1AK3
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
| Amp100, Kan25
| Default double transcriptional terminator
|
|
| iGEM strain box 1
| 26
| rfp
| DH5α
| pSB2K3
| E1010
| Kan25
| Red fluorescent protein (shows up pink even without appropriate wavelength of light and filter)
|
|
| iGEM strain box 1
| 27
| Pseudomonas mendocina
| kr 1
|
|
| ?
| Wrong strain for cloning PmeI (received from Paul Hatzinger at Shaw Environmental NJ). Degrades N-nitrodimethylamine (NDMA). Appl Environ Microbiol. 2006 Oct;72(10):6693-8. Epub 2006 Sep 1
| 2008:08:xx
|
| iGEM strain box 1
| 28
| Plac
| DH5α
| pSB1A2
| [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
| Amp100
| Wildtype LacI repressable promoter
|
|
| iGEM strain box 1
| 29
| PtetR
| DH5α
| pSB1A2
| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
| Amp100
| Promoter that is repressed by tetR and derepressed by tetracyclin.
|
|
| iGEM strain box 1
| 30
| CI
| DH5α
| pSB1A2
| [http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
| Amp100
| CI repressor for λ Pr promoter
|
|
| iGEM strain box 1
| 31
| rTT
| DH5α
| pSB1A2
| B0025
| Amp100
| Reverse transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015])
| 2008:xx:xx
|
| iGEM strain box 1
| 32
| Taq
| DH5α
|
|
| Amp100
| Taq strain from Moffatt lab for purifying taq
| 2008:08:xx
|
| iGEM strain box 1
| 33
| Pconst+
| DH5α
| pSB1A2
| [http://partsregistry.org/Part:BBa_J23119 BBa_J23119]
| Amp100
| Wildtype promoter─strongest of this promoter family ([http://partsregistry.org/Part:BBa_J23100 BBa_J23100]-[http://partsregistry.org/Part:BBa_J23119 BBa_J23119])
|
|
| iGEM strain box 1
| 34
| LacI
| DH5α
| pSB1A2
| [http://partsregistry.org/Part:BBa_C0012 BBa_C0012]
| Amp100
| LacI repressor for Plac Promoter ([http://partsregistry.org/Part:BBa_R0010 BBa_R0010]) with LVA degredation tag
|
|
| iGEM strain box 1
| 35
| Pconst4%
| DH5α
| [http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
| [http://partsregistry.org/Part:BBa_J23109 BBa_J23109]
| Amp100
| Constitutive promoter. Expression level is 4% in comparison with the highest expression levels from this Pconst family (ex. wildtype promoter)
|
|
| iGEM strain box 1
| 36
| TTbi
| DH5α
| pSB1AK3
| [http://partsregistry.org/Part:BBa_B0014 BBa_B0014]
| Amp100,Kan25
| Double terminator─not as strong as [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] but more even efficiency for forward and reverse use.
|
|
| iGEM strain box 1
| 37
| Pconst100%
| DH5α
| [http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
| [http://partsregistry.org/Part:BBa_J23100 BBa_J23100]
| Amp100
| Constitutive promoter. Expression level is 100%─the highest expression level for this Pconst family (i.e., wildtype promoter)
|
|
| iGEM strain box 1
| 38
| PlacCI
| DH5α
| pSB2K3
|
| Kan25
| cI repressor ([http://partsregistry.org/Part:BBa_C0051 BBa_C0051]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) under the promoter Plac ([http://partsregistry.org/Part:BBa_R0011 BBa_R0011]).
| 2009:04:xx
|
| iGEM strain box 1
| 39
| pTC300
| DH5α
| pUC19
|
| Cm10, Kan25
| Strain containing Kanamycin resistance gene, and SacB negative selection gene flanked together by BamHI sites
| 2009:05:30
|
| iGEM strain box 1
| 40
| DB 3.1
| DB 3.1
|
|
|
| CCDB resistant strain, contains mutation in GyrA
| 2009:05:30
|
| iGEM strain box 1
| 41
| DH5α
| DH5 alpha
|
|
|
| For making competent cells. Default strain for
| 2007:xx:xx
|
| iGEM strain box 1
| 42
| pFB9006
| DH5α λ pir
| pFB9001
|
| Strep100/Kan20
| pFB9005+KanSac. pFB9001 containing H2 homologous region to E. coli melA gene. Does not contain H1 region.
| 2009:06:xx
|
| iGEM strain box 1
| 43
| pSB1A3
| DH5α
| pSB1A3
|
| Amp 100
| BioBrick vector from 2009 registry.
| 2009:06:16
|
| iGEM strain box 1
| 44
| luxI
| DH5α
| pSB1AK3
|
| Amp100, Kan 25??
| luxI ([http://partsregistry.org/Part:BBa_C0061 BBa_C0061]) +RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and transcriptional terminator ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015])
| 2007:xx:xx
|
| iGEM strain box 1
| 45
| PlaclasR
| DH5α
| pSB1A2
|
| Amp100
| lasR (C0079) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and TT ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015]) under the control of a lac promoter (unsure which promoter - would need testing/sequencing)
| 2007:xx:xx
|
| iGEM strain box 1
| 46
| Plux rfp'
| DH5α
| pSB2K3
|
| Kan25
| RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) under Plux promoter
| 2007:xx:xx
|
| iGEM strain box 1
| 47
| Plas rfp'
| DH5α
| pSB2K3
|
| Kan25
| RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) under Plas promoter ([http://partsregistry.org/Part:BBa_R0079 BBa_R0079])
| 2007:xx:xx
|
| iGEM strain box 1
| 48
| Ptet rfp'
| DH5α
| pSB2K3
|
| Kan25
| RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) under Ptet promoter ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040])
| 2007:xx:xx
|
| iGEM strain box 1
| 49
| PompR rfp'
| DH5α
| pSB2K3
|
| Kan25
| RFP ([http://partsregistry.org/Part:BBa_E1010 BBa_E1010]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) under PompR promoter
| 2007:xx:xx
|
| iGEM strain box 1
| 50
| tetR
| DH5α
| pSB2K3
|
| Kan25
| tetR repressor ([http://partsregistry.org/Part:BBa_C0040 BBa_C0040]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) and TT ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015])
| 2007:xx:xx
|
| iGEM strain box 1
| 51
| ???
| DH5α
| pSB2K3
|
| Kan25
|
|
|
| iGEM strain box 1
| 52
| lasI
| DH5α
| pSB2K3
|
| Kan25
| lasI ([http://partsregistry.org/Part:BBa_C0078 BBa_C0078]) with RBS ([http://partsregistry.org/Part:BBa_E0034 BBa_E0034]) and TT ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015])
| 2007:xx:xx
|
| iGEM strain box 1
| 53
| tetR
| DH5α
| pSB2K3
|
| Kan25
| tetR repressor ([http://partsregistry.org/Part:BBa_C0040 BBa_C0040]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) and TT ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015])
| 2007:xx:xx
|
| iGEM strain box 1
| 54
| tetR'
| DH5α
| pSB1A2
|
| Amp100
| tetR repressor ([http://partsregistry.org/Part:BBa_C0040 BBa_C0040]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034])
| 2007:xx:xx
|
| iGEM strain box 1
| 55
| lasR
| DH5α
| pSB1A2
|
| Amp100
| lasR ([http://partsregistry.org/Part:BBa_C0079 BBa_C0079]) with RBS ([http://partsregistry.org/Part:BBa_B0034 BBa_B0034]) and TT ([http://partsregistry.org/Part:BBa_B0015 BBa_B0015])
| 2007:xx:xx
|
| iGEM strain box 1
| 56
| Plac IA7K
| DH5α
| pSB1A2
|
| Amp100
| ????
|
|
| iGEM strain box 1
| 57
| pSB1A2 attB TG
| DH5α
| pSB1A2
|
| Amp100
| Was previously Pr RFP', RFP was cut out and attB TG was inserted. Flanked by EcoRI and PstI sites.
| 2009:07:06
|
| iGEM strain box 1
| 58
| pSB1A2 attB TT
| DH5α
| pSB1A2
|
| Amp100
| Same as Strain #57, except the inserted fragment was attB TT instead. This is the wild-type attB.
| 2009:07:06
|
| iGEM strain box 1
| 59
| pSB1A2 attB CT
| DH5α
| pSB1A2
|
| Amp100
| Same as Strain #57, except the inserted fragment was attB CT instead.
| 2009:07:06
|
| iGEM strain box 1
| 60
| rPlacTT + PlacCI
| DH5α
| pSB2K3
|
|
|
| 2009:06:15
|
| iGEM strain box 1
| 61
| 2008 Construct w/o Promoter
| DH5α
| pSB1A2
|
| Amp100
| RBS + Pmel + rPlacTT + PlacCI. 2008 project.
| 2009:07:10
|
| iGEM strain box 1
| 62
| 2008 Constant
| DH5α
|
|
| Amp100
|
| 2009:07:15
|
| iGEM strain box 1
| 63
| pSB3C5
| DB3.1
| pSB3C5
|
| Cm25
| Low copy # plasmid, no promoter, no RBS, "CCDD" (BBa 15001/15002)?
|
|
| iGEM strain box 1
| 64
| BBa
|
| pSB3C5
|
|
|
|
|
| iGEM strain box 1
| 65
| pKNG101
| DH5α
| pKNG101
|
| Strep100
| Plasmid backbone for the pFB9xxx series.
| 2009:08:07
|
| iGEM strain box 1
| 66
| DH5α λ pir
| DH5 alpha lambda pir
| None
|
| None
| DH5α λ pir stock for making competent cells.
| 2009:07:24
|
| iGEM strain box 1
| 67
| pFB9007
| DH5α λ pir
| pFB9001
|
| Strep100
| Contains H2 region and an attP site. Same as pFB9004 except w/o the truncated SacB gene on the plasmid backbone.
| 2009:08:05
|
| iGEM strain box 1
| 68
| pFB9008
| DH5α λ pir
| pFB9007
|
| Strep100
| Same as pFB9007, but it has RFP cloned in upstream of the attP site and the H2 region.
| 2009:08:05
|
| iGEM strain box 1
| 69
| pFB9009 #2
| DH5α λ pir
| pFB9008+H1
|
| Strep100/Kan20
| Same as pFB9008+H2, but it has Kanr and sacB genes cloned in between the H1&H2 regions, directly downstream adjacent to the RFP.
| 2009:08:28
|
| iGEM strain box 1
| 70
|
|
|
|
|
|
|
|
| iGEM strain box 1
| 71
| pSB4A5
| DH5α
| pSB4A5
|
| Amp100
| Low copy number, no promoter, no RBS.
| 2009:08:28
|
| iGEM strain box 1
| 72
| pSB4C5
| DB3.1
| pSB4C5
|
| Cm25
| Low copy number, no promoter, no RBS.
| 2009:08:28
|
| iGEM strain box 1
| 73
| OriT-L #13
| DH5α
| pSB1A2
|
| Amp100
|
| 2009:08:28
|
| iGEM strain box 1
| 74
| OriT-L #18
| DH5α
| pSB1A2
|
| Amp100
|
| 2009:08:28
|
| iGEM strain box 1
| 75
| [http://partsregistry.org/Part:BBa_J23116 BBa_J23116] Pconst
| DH5α
| [http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
|
| Amp100
|
| 2009:08:28
|
| iGEM strain box 1
| 76
| [http://partsregistry.org/Part:BBa_J23118 BBa_J23118] Pconst
| DH5α
| [http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
|
| Amp100
|
| 2009:08:28
|
| iGEM strain box 1
| 77
| pFB9008+H1 #13
| DH5α λ pir
| pFB9007
|
| Strep100/Kan20
| Same as pFB9008, except that H1 region and its attP site are cloned in upstream of the RFP gene to flank the RFP. Low copy number.
| 2009:08:28
|
| iGEM strain box 1
| 78
| pSB4C5-1
| DB3.1
| pSB4C5
|
| Cm25
|
| 2009:08:20
|
| iGEM strain box 1
| 79
| pSB4C5-2
| DB3.1
| pSB4C5
|
| Cm25
|
| 2009:08:20
|
"
