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Template:Team:KULeuven/28 September 2009/VanillinProduction
From 2009.igem.org
- Miniprep of PEFT, PAMSCO electroporation, PAMSCOEFT electroporation
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| PEFT1 | 240,3 | ||
| PEFT2 | 169,6 | ||
| PAMSCO1 | 184,3 | ||
| PAMSCO2 | 184,5 | ||
| PAMSCOT1 | 232,5 | ||
| PAMSCOT2 | 176,6 | ||
| PAMSCOT3 | 293,8 | ||
| PAMSCOT4 | 123,1 | ||
| PAMSCOEFT1 | 87,4 | ||
| PAMSCOEFT2 | 206,9 | ||
| PAMSCOEFT3 | 218,4 | ||
| PAMSCOEFT4 | 188,2 |
- Restriction digest with E/S
- PAMSCOT has the right length!
- PAMSCOEFT appears to be a self-closed Terminator vector
- Transfered an additional 6 colonies from PAMSCOEFT to another plate and liquid cultures
