Virginia Commonwealth/12 June 2009

Friday 12 June 2009

Data from plasmid purification (Miniprep)
- Buffer EB was used instead of water and 500 microliters was used instead of 50 microliters so the DNA will have to be concentrated.
- 114 microliters of buffer and 6 microliters of sample (20x dilution)


Part No.	A260 (nm)	A280 (nm)	A260/A280	Conc. (µg/mL)	Amount (µg)	Volume (µL)
J23103	.007	.003	2.33	7.0	2.52	360
J23105	.008	.004	2.00	8.0	2.96	370
J23100	.014	.011	1.273	14.0	5.32	380
J23104	.011	.009	1.22	11.0	4.18	380
J23101	.012	.008	1.5	12.0	4.56	380
J23107	.018	.014	1.286	18.0	8.1	450
J23116	.020	.016	1.25	20.0	7.2	360
J23113	.014	.010	1.4	14.0	5.18	370
J23110	.012	.007	1.714	12.0	4.2	350


Part No.	A260 (nm)	A280 (nm)	A260/A280	Concentration (µg/mL)	Amount (µg)	Volume (µL)
J23102	.031	.025	1.24	31.0	10.23	330
R0011	.021	.016	1.313	21.0	5.46	260
R0040	.015	.011	1.364	15.0	5.4	360

MandM 19:17, 12 June 2009 (UTC)

Mini-Prep on the promoter.
Maxi-Prep on the reporter and the backbone.
- Maxi-Prep yielded no DNA so the cells were re-cultivated overnight to perform 4 mini-preps on them tomorrow. All cells were re-cultivated over night to prepare for freezing.

MandM 19:01, 12 June 2009 (UTC)

Bussingkm 15:40, 13 June 2009 (UTC)