From 2009.igem.org
Monday 27 July 2009
Results
Kevin & Adam
Results from Friday's PCR product
| ## | A260 | Volume (uL) | Concentration (ug/uL) | Amount (ug)
|
| Design 1 0.0 | .017 | 40.1 | .034 | 1.36
|
| Design 1 0.5 | .018 | 40.1 | .036 | 1.44
|
| Design 1 1.0 | .014 | 40.0 | .028 | 1.12
|
| Design 1 1.5 | .012 | 40.4 | .024 | .97
|
| Design 2 | .047 | 39.4 | .094 | 3.7
|
| Design 3 | .028 | 41.0 | .056 | 2.29
|
| Design 4 | .039 | 48.0 | .078 | 3.74
|
| Design 5 | .032 | 36.0 | .064 | 2.30
|
| Design 6 | .030 | 39.7 | .060 | 2.38
|
| Design 7 | .029 | 43.0 | .058 | 2.49
|
| Design 8 | .022 | 39.2 | .044 | 1.72
|
| Design 9 | .032 | 41.9 | .064 | 2.68
|
For Digestion and Ligation of Promoters
| ## | A260 | Volume (uL) | Concentration (ug/uL) | Amount (ug)
|
| pSB4C5 | .086 | 19.5 | .172 | 3.18
|
| J06702 | .023 | 125.0 | .040 | 5.75
|
| BBa_J23101 | .052 | 35.2 | .104 | 3.66
|
Maria and Afton
- Nothing is glowing except one colony, J23110 w/ 702
Trentay 16:57, 28 July 2009 (UTC)
Tasks
Kevin & Adam
- Discuss and continue to study design principles for T7 and various other inducible/repressible promoters.
Maria and Afton
- Regrow, Miniprep, Take spectrophotometry measurements, Digest, Ligate, Transform ligated parts:
- Troubleshoot and Repeat this process until RFP is expressed
- Make overnight cultures of parts pSB4C5, J23110, J06702, J23110 w/ 702
Trentay 16:57, 28 July 2009 (UTC)
Wetlab
Kevin & Adam
- Digest and Ligate synthetic promoter designs 1-9.
Maria and Afton
- Overnight cultures were made (5mL of selective broth with 100 microliters of DNA)
- Samples taken from cryogenic stocks
- J23110 (AMP)
- J06702 (AMP)
- pSB4C5 (Cm)
- Samples taken from plates
Trentay 16:57, 28 July 2009 (UTC)
|
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