Team:Newcastle/SporulationTuning

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==References==
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[1] Veening, J-W., Smits, W. K., Kuipers, O. P. (2008) Bistability, Epigenetics, and Bet-Hedging in Bacteria. Annu. Rev. Microbiol. 62: 193-210
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[2] Fujita, M., Losick, R. (2005) Evidence that Entry into Sporulation in Bacillus subtilis is Governed by a Gradual Increase in the Level and Activity of the Master Regulator Spo0A. 19: 2236–2244
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[3] Sonenshein, A.L., Hoch, J.A., Losick, R., (2002) Bacillus subtilis and Its Closest Relatives From Genes to Cells. ASM Press, United States of America. Pp 476–477
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Revision as of 14:14, 15 October 2009


Sporulation Tuning

Introduction

In this section of our project, we hope to control sporulation in our bacterial population, such that we can decide how much of the population becomes spores, and how much continue as vegetative cells. Should the cell sporulate, it would become a ‘metal container’, trapping the sequestered cadmium in its spore.

After the cell sequesters cadmium into its spore, it should not germinate or the sequestered cadmium will be released back into the environment as a result. Therefore, the role of chassis comes into play, where the sleB and cwlJ germination-defective mutants are put into use. More information about this other sub-project of ours can be found here.

Novelty in this sub-project

Instead of allowing the cell to decide whether or not to sporulate, we hope to influence it's decision. We plan to use kinA as a part of this system.

Modelling

See Team:Newcastle/Modeling/Tuning

KinA Expression Model Results

TeamNewcastleKinAExpressionIptg1000.png


TeamNewcastleKinAExpressionIptg3000.png


TeamNewcastleKinAExpressionIptg5000.png


TeamNewcastleKinAExpressionIptg8000.png


TeamNewcastleKinAExpressionIptg10000.png

BioBrick constructs

A BioBrick which we are designing is to contain an IPTG inducable kinA gene, using pSpac, allowing us to test the theory about kinA in the lab.

Lab Work Strategies

The lab work will mainly be to test our BioBrick using IPTG.

Other Presentations and Diagrams

References

[1] Veening, J-W., Smits, W. K., Kuipers, O. P. (2008) Bistability, Epigenetics, and Bet-Hedging in Bacteria. Annu. Rev. Microbiol. 62: 193-210

[2] Fujita, M., Losick, R. (2005) Evidence that Entry into Sporulation in Bacillus subtilis is Governed by a Gradual Increase in the Level and Activity of the Master Regulator Spo0A. 19: 2236–2244

[3] Sonenshein, A.L., Hoch, J.A., Losick, R., (2002) Bacillus subtilis and Its Closest Relatives From Genes to Cells. ASM Press, United States of America. Pp 476–477




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