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Template:Team:KULeuven/31 August 2009/VanillinReceptor
From 2009.igem.org
(Difference between revisions)
(New page: * TOPO cloning showed only blue colonies so we searched for alternatives. We did resaerchwork if it was maybe possible with a puc-vector * The mutation of rpoA was again checked but gelele...) |
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* The mutation of rpoA was again checked but gelelectrophoresis showed no result | * The mutation of rpoA was again checked but gelelectrophoresis showed no result | ||
* X,Y and K (the igemvector) were cut with EcoR1 and Pst1. | * X,Y and K (the igemvector) were cut with EcoR1 and Pst1. | ||
| - | * new ligation was done with the first cuttingproduct. | + | * new ligation was done with the first cuttingproduct so today it was electroporated and plated. |
* PCR was done for W to get more basicproduct to work with | * PCR was done for W to get more basicproduct to work with | ||
Latest revision as of 12:28, 17 October 2009
- TOPO cloning showed only blue colonies so we searched for alternatives. We did resaerchwork if it was maybe possible with a puc-vector
- The mutation of rpoA was again checked but gelelectrophoresis showed no result
- X,Y and K (the igemvector) were cut with EcoR1 and Pst1.
- new ligation was done with the first cuttingproduct so today it was electroporated and plated.
- PCR was done for W to get more basicproduct to work with
